MASSIVE CLUSTER IMPACT MASS-SPECTROMETRY - A NEW DESORPTION METHOD FOR THE ANALYSIS OF LARGE BIOMOLECULES

被引：94

作者：

MAHONEY, JF ^{[1
]}

PEREL, J ^{[1
]}

RUATTA, SA ^{[1
]}

MARTINO, PA ^{[1
]}

HUSAIN, S ^{[1
]}

LEE, TD ^{[1
]}

机构：

[1] CITY HOPE NATL MED CTR,BECKMAN RES INT,DIV IMMUNOL,DUARTE,CA 91010

来源：

RAPID COMMUNICATIONS IN MASS SPECTROMETRY | 1991年 / 5卷 / 10期

关键词：

D O I：

10.1002/rcm.1290051004

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A new ion desorption method is described that utilizes a primary beam of massive, multiply charged cluster ions to generate secondary ions of peptides in a glycerol matrix. The massive cluster ion beam is generated via electrohydrodynamic emission using a 1.5 M solution of ammonium acetate in 30% aqueous glycerol. Negative ion spectra of peptides obtained using this technique show greatly decreased relative intensities for fragment ions and 'chemical noise' background when compared to spectra obtained using a xenon atom primary beam. The near absence of fragments derived from radiation damage to the sample solution is attributed to the impact of primary particles with energies less than 1 eV/nucleon.

引用

页码：441 / 445

页数：5

共 37 条

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