PROSPECTS FOR CONFOCAL IMAGING BASED ON NANOSECOND FLUORESCENCE DECAY TIME

被引：27

作者：

MORGAN, CG

MITCHELL, AC

MURRAY, JG

机构：

[1] Department of Biological Sciences, University of Salford, Salford

来源：

JOURNAL OF MICROSCOPY | 1992年 / 165卷

关键词：

FLUORESCENCE DECAY TIME; PHASE SHIFT DEMODULATION FLUOROMETRY; FLUORESCENCE IMAGING;

D O I：

10.1111/j.1365-2818.1992.tb04304.x

中图分类号：

TH742 [显微镜];

学科分类号：

摘要：

A fluorescence microscope has been developed to give contrast on the basis of fluorescence decay time. Two different detection schemes are used, one based on an imaging single photon detector equipped with an external photon correlator and the other using an RF-modulated intensified CCD camera. Examples of the new contrast are shown, and the possible routes to development of a confocal version of the microscope are discussed.

引用

页码：49 / 60

页数：12

共 19 条

[11]

MORGAN CG, 1990, T ROY MICR, V1, P463

[12]

MORGAN CG, 1990, P SOC PHOTO-OPT INS, V1204, P798, DOI 10.1117/12.17773

[13]

MORGAN JG, 1991, J PHYS E, V179, P211

[14]

OConnor D. V. O., 1984, TIME CORRELATED SING

[15] THE TANDEM SCANNING REFLECTED LIGHT-MICROSCOPE [J].

PETRAN, M ;

HADRAVSKY, M ;

BOYDE, A .

SCANNING, 1985, 7 (02) :97-108

[16] MINIATURE IMAGING PHOTON DETECTORS .2. DEVICES WITH TRANSPARENT PHOTO-CATHODES [J].

REES, D ;

MCWHIRTER, I ;

ROUNCE, PA ;

BARLOW, FE .

JOURNAL OF PHYSICS E-SCIENTIFIC INSTRUMENTS, 1981, 14 (02) :229-233

[17]

TIAN R, 1991, TOPICS MOL STRUCTURA, V15, P177

[18] IMAGING IN SCANNING MICROSCOPES WITH SLIT-SHAPED DETECTORS [J].

WILSON, T ;

HEWLETT, SJ .

JOURNAL OF MICROSCOPY-OXFORD, 1990, 160 :115-139

[19]

Wilson T, 1984, THEORY PRACTICE SCAN

← 1 2 →