SYNTHESIS AND APPLICATIONS OF OLIGORIBONUCLEOTIDES WITH SELECTED 2'-O-METHYLATION USING THE 2'-O-[1-(2-FLUOROPHENYL)-4-METHOXYPIPERIDIN-4-YL] PROTECTING GROUP

被引：51

作者：

BEIJER, B ^{[1
]}

SULSTON, I ^{[1
]}

SPROAT, BS ^{[1
]}

RIDER, P ^{[1
]}

LAMOND, AI ^{[1
]}

NEUNER, P ^{[1
]}

机构：

[1] EUROPEAN MOLEC BIOL LAB,POSTFACH 102209,W-6900 HEIDELBERG,GERMANY

来源：

NUCLEIC ACIDS RESEARCH | 1990年 / 18卷 / 17期

关键词：

D O I：

10.1093/nar/18.17.5143

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The synthesis of base protected 5′-O-dimethoxytrityl-2′-O-[1-(2-fluorophenyl)-4-methoxypiperidin-4-yl]-3′-O-(2-cyanoethyl N,N-diisopropylphosphoramidites) is described, using phenoxyacetyl protection for the exocyclic amino groups of guanosine and adenosine and acetyl protection of the amino group of cytidine. High yield assembly of these building blocks into oligoribonucleotides on aminopropyl controlled pore glass was achieved using 5-(4-nitrophenyl)-1H-tetrazole as activator. Mixed sequences containing selected 2′-O-methylation were also synthesised and their significance for the study of RNA biochemistry is discussed. © 1990 Oxford University Press.

引用

页码：5143 / 5151

页数：9

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