INDUCTION OF MINISATELLITE DNA REARRANGEMENTS BY GENOTOXIC CARCINOGENS IN MOUSE-LIVER TUMORS

被引：23

作者：

LEDWITH, BJ ^{[1
]}

JOSLYN, DJ ^{[1
]}

TROILO, P ^{[1
]}

LEANDER, KR ^{[1
]}

CLAIR, JH ^{[1
]}

SOPER, KA ^{[1
]}

MANAM, S ^{[1
]}

PRAHALADA, S ^{[1
]}

VANZWIETEN, MJ ^{[1
]}

NICHOLS, WW ^{[1
]}

机构：

[1] RUTGERS STATE UNIV, DEPT BIOL, CAMDEN, NJ 08102 USA

来源：

CARCINOGENESIS | 1995年 / 16卷 / 05期

关键词：

D O I：

10.1093/carcin/16.5.1167

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

We investigated whether somatic rearrangements in minisatellite DNA are more frequent in chemically induced mouse liver tumors than they are in spontaneous tumors. CD-1 mouse liver tumors were induced by either a single dose or 15 consecutive daily doses of 7,12-dimethylbenz[a]anthracene, 4-aminoazobenzene, N-hydroxy-2-acetyl-aminofluorene or diethylnitrosoamine (DEN). Using DNA fingerprinting analysis, we found that the single- and multiple-dose carcinogen treatments caused a 2- to 5-fold higher frequency of minisatellite DNA rearrangements compared with that found in spontaneous tumors-with the exception of single-dose DEN tumors, which showed no increase in rearrangements. Our results suggest that DNA fingerprinting may be a valuable assay for differentiating certain chemically induced tumors from spontaneous tumors.

引用

页码：1167 / 1172

页数：6

共 45 条

[1] ANALYSIS OF SOMATIC MUTATIONS AT HUMAN MINISATELLITE LOCI IN TUMORS AND CELL-LINES [J].