CHARACTERIZATION OF A HIGHLY POLYMORPHIC REGION NEAR THE 1ST EXON OF THE HUMAN MAOA GENE CONTAINING A GT DINUCLEOTIDE AND A NOVEL VNTR MOTIF

被引：62

作者：

HINDS, HL ^{[1
]}

HENDRIKS, RW ^{[1
]}

CRAIG, IW ^{[1
]}

CHEN, ZY ^{[1
]}

机构：

[1] UNIV OXFORD,DEPT BIOCHEM,GENET LAB,OXFORD OX1 3QU,ENGLAND

来源：

GENOMICS | 1992年 / 13卷 / 03期

关键词：

D O I：

10.1016/0888-7543(92)90181-Q

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The genes encoding the A and B forms of the human monoamine oxidase enzymes (MAOA and MAOB) are localized at Xp11.23-Xp11.4. We report the characterization of a highly informative polymorphic region within a 2.9-kb cloned fragment containing the first exon of the MAOA gene. The polymorphic region consists of a GT microsatellite directly adjacent to an imperfectly duplicated novel 23-bp VNTR motif. DNA sequencing within and flanking the repeated segment allowed the design of specific amplification primers. In 56 unrelated females, 15 different alleles were identified with sizes ranging from 285 to 388 bp. The alleles differed in both the number of dinucleotide and the number of VNTR repeats, yielding a highly informative polymorphic marker locus with a calculated heterozygosity value of 75%. © 1992.

引用

页码：896 / 897

页数：2

共 15 条

[1] CDNA CLONING OF HUMAN-LIVER MONOAMINE OXIDASE-A AND OXIDASE-B - MOLECULAR-BASIS OF DIFFERENCES IN ENZYMATIC-PROPERTIES [J].

BACH, AWJ ;

LAN, NC ;

JOHNSON, DL ;

ABELL, CW ;

BEMBENEK, ME ;

KWAN, SW ;

SEEBURG, PH ;

SHIH, JC .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (13) :4934-4938

[2]

BLACK GCM, 1991, NUCLEIC ACIDS RES, V19, P689

[3] STRUCTURE OF THE HUMAN GENE FOR MONOAMINE-OXIDASE TYPE-A [J].

CHEN, ZY ;

HOTAMISLIGIL, GS ;

HUANG, JK ;

WEN, L ;

EZZEDDINE, D ;

AYDINMUDERRISOGLU, N ;

POWELL, JF ;

HUANG, RH ;

BREAKEFIELD, XO ;

CRAIG, I ;

HSU, YPP .

NUCLEIC ACIDS RESEARCH, 1991, 19 (16) :4537-4541

[4] HUMAN MONOAMINE OXIDASE-A AND OXIDASE-B GENES EXHIBIT IDENTICAL EXON INTRON ORGANIZATION [J].

GRIMSBY, J ;

CHEN, K ;

WANG, LJ ;

LAN, NC ;

SHIH, JC .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (09) :3637-3641

[5]

HOTAMISLIGIL GS, 1991, AM J HUM GENET, V49, P383

[6] MOLECULAR-GENETICS OF THE MONOAMINE OXIDASES [J].

HSU, YPP ;

POWELL, JF ;

SIMS, KB ;

BREAKEFIELD, XO .

JOURNAL OF NEUROCHEMISTRY, 1989, 53 (01) :12-18

[7] LOCALIZATION OF HUMAN MONOAMINE OXIDASE-A GENE TO XP11.23-11.4 BY INSITU HYBRIDIZATION - IMPLICATIONS FOR NORRIE DISEASE [J].

LEVY, ER ;

POWELL, JF ;

BUCKLE, VJ ;

HSU, YPP ;

BREAKEFIELD, XO ;

CRAIG, IW .

GENOMICS, 1989, 5 (02) :368-370

[8] VARIABLE NUMBER OF TANDEM REPEAT (VNTR) MARKERS FOR HUMAN-GENE MAPPING [J].

NAKAMURA, Y ;

LEPPERT, M ;

OCONNELL, P ;

WOLFF, R ;

HOLM, T ;

CULVER, M ;

MARTIN, C ;

FUJIMOTO, E ;

HOFF, M ;

KUMLIN, E ;

WHITE, R .

SCIENCE, 1987, 235 (4796) :1616-1622

[9]

RILEY S, 1991, DEGENERATIVE RETINOPATHIES : ADVANCES IN CLINICAL AND GENETIC RESEARCH, P119

[10] PRIMER-DIRECTED ENZYMATIC AMPLIFICATION OF DNA WITH A THERMOSTABLE DNA-POLYMERASE [J].

SAIKI, RK ;

GELFAND, DH ;

STOFFEL, S ;

SCHARF, SJ ;

HIGUCHI, R ;

HORN, GT ;

MULLIS, KB ;

ERLICH, HA .

SCIENCE, 1988, 239 (4839) :487-491

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