PYRUVIC-ACID PRODUCTION BY A LIPOIC ACID AUXOTROPH OF ESCHERICHIA-COLI W1485

被引：8

作者：

YOKOTA, A

SHIMIZU, H

TERASAWA, Y

TAKAOKA, N

TOMITA, F

机构：

来源：

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | 1994年 / 41卷 / 06期

关键词：

D O I：

暂无

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A lipoic acid auxotroph of Escherichia coli K-12, strain W1485lip2 (ATCC25645), produced pyruvic acid aerobically from glucose under the lipoic acid-deficient conditions, while the prototrophic parent strain, W1485 (ATCC12435), produced 2-oxoglutaric acid as the main product. The mechanism of the pyruvic acid production by strain W1485lip2 was found to be the impaired oxidative decarboxylation of pyruvic acid caused by the decrease in the activity of pyruvate dehydrogenase complex under the conditions of lipoic acid deficiency. Under the optimum culture conditions using the pH-controlled jar fermentor, 25.5 g/l pyruvic acid was obtained from 50 g/l glucose after the culture for 32-40 h at pH 6.0. The relationship between the pyruvic acid productivity and the pyruvate dehydrogenase complex activity in jar-fermenter culture was discussed.

引用

收藏

页码：638 / 643

页数：6

相关论文

共 12 条

[1] BIOCHEMICAL AND GENETIC STUDIES WITH LYSINE + METHIONINE MUTANTS OF ESCHERICHIA COLI - LIPOIC ACID AND ALPHA-KETOGLUTARATE DEHYDROGENASE-LESS MUTANTS [J].

HERBERT, AA ;

GUEST, JR .

JOURNAL OF GENERAL MICROBIOLOGY, 1968, 53 :363-+

[2]

MALDONADO ME, 1972, J BIOL CHEM, V247, P2711

[3] BACTERIAL SYNTHESIS OF L-TRYPTOPHAN AND ITS ANALOGS .1. SYNTHESIS OF L-TRYPTOPHAN FROM PYRUVATE, AMMONIA AND INDOLE [J].

NAKAZAWA, H ;

ENEI, H ;

OKUMURA, S ;

YAMADA, H .

AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1972, 36 (13) :2523-2528

[4] ENZYMATIC PREPARATION OF L-TRYPTOPHAN AND 5-HYDROXY-L-TRYPTOPHANAN [J].

NAKAZAWA, H ;

OKUMURA, S ;

YAMADA, H ;

YOSHIDA, H ;

ENEI, H .

FEBS LETTERS, 1972, 25 (01) :43-&

[5] ENZYMATIC PRODUCTION OF TRYPTOPHAN USING A LIPOIC ACID AND THIAMINE DOUBLE AUXOTROPH OF ENTEROBACTER-AEROGENES HAVING BOTH PYRUVIC-ACID PRODUCTIVITY AND HIGH TRYPTOPHANASE ACTIVITY [J].

OITA, S ;

YOKOTA, A ;

TAKAO, S .

JOURNAL OF FERMENTATION AND BIOENGINEERING, 1990, 69 (04) :256-258

[6] LIPOIC ACID METABOLISM IN ESCHERICHIA-COLI - SEQUENCING AND FUNCTIONAL-CHARACTERIZATION OF THE LIPA AND LIPB GENES [J].

REED, KE ;

CRONAN, JE .

JOURNAL OF BACTERIOLOGY, 1993, 175 (05) :1325-1336

[7]

TAKAO S, 1984, J FERMENT TECHNOL, V62, P329

[8] LIPOIC ACID METABOLISM IN ESCHERICHIA-COLI - ISOLATION OF NULL MUTANTS DEFECTIVE IN LIPOIC ACID BIOSYNTHESIS, MOLECULAR-CLONING AND CHARACTERIZATION OF THE ESCHERICHIA-COLI LIP LOCUS, AND IDENTIFICATION OF THE LIPOYLATED PROTEIN OF THE GLYCINE CLEAVAGE SYSTEM [J].

VANDENBOOM, TJ ;

REED, KE ;

CRONAN, JE .

JOURNAL OF BACTERIOLOGY, 1991, 173 (20) :6411-6420

[9] SYNTHESIS OF L-TYROSINE FROM PYRUVATE, AMMONIA AND PHENOL BY CRYSTALLINE TYROSINE PHENOL LYASE [J].

YAMADA, H ;

TORII, H ;

ENEI, H ;

KASHIMA, N ;

OKUMURA, S ;

KUMAGAI, H .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1972, 46 (02) :370-&

[10] TRYPTOPHAN PRODUCTION BY A LIPOIC ACID AUXOTROPH OF ENTEROBACTER-AEROGENES HAVING BOTH PYRUVIC-ACID PRODUCTIVITY AND HIGH TRYPTOPHANASE ACTIVITY [J].

YOKOTA, A ;

OITA, S ;

TAKAO, S .

AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1989, 53 (08) :2037-2044