HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY OF RAW SUGARS AND POLYOLS USING BONDED SILICA-GELS

被引：38

作者：

HERBRETEAU, B ^{[1
]}

LAFOSSE, M ^{[1
]}

MORINALLORY, L ^{[1
]}

DREUX, M ^{[1
]}

机构：

[1] UNIV ORLEANS, CHIM BIOORGAN & ANALYT LAB, CNRS, URA 499, BP 6759, F-45067 ORLEANS 2, FRANCE

来源：

CHROMATOGRAPHIA | 1992年 / 33卷 / 7-8期

关键词：

COLUMN LIQUID CHROMATOGRAPHY; DIOL SILICA GELS; CARBOHYDRATES; EVAPORATIVE LIGHT SCATTERING DETECTION;

D O I：

10.1007/BF02275911

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Bonded silica columns have been evaluated for their ability to separate carbohydrates and polyols. Mobile phases consisting of dichloromethane/methanol produced the best separations in comparison with the acetonitrile/water mixtures commonly used with amino columns. Of all the bonded phases tested, LiChrospher Diol silica provided the best separations, and selectivities were not very different from those obtained on the most popular system using an amino bonded phase and acetonitrile/water as eluent. In addition, diol columns with a dichloromethane/methanol eluent offer excellent stability with no Schiff's base formation of reducing sugars. Using an evaporative light scattering detector, low limit detection is obtainable (20 ng of glucose from a column) and gradient elution is quite feasible.

引用

页码：325 / 330

页数：6

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