CALCIUM-SENSITIVE CONTROL OF RHODOPSIN PHOSPHORYLATION IN THE RECONSTITUTED SYSTEM CONSISTING OF PHOTORECEPTOR-MEMBRANES, RHODOPSIN KINASE AND RECOVERIN

被引：54

作者：

GORODOVIKOVA, EN ^{[1
]}

SENIN, II ^{[1
]}

PHILIPPOV, PP ^{[1
]}

机构：

[1] MOSCOW MV LOMONOSOV STATE UNIV,DEPT ENZYMOL,MOSCOW 119899,RUSSIA

来源：

FEBS LETTERS | 1994年 / 353卷 / 02期

基金：

俄罗斯基础研究基金会;

关键词：

PHOTORECEPTION; PHOSPHORYLATION; RHODOPSIN; RHODOPSIN KINASE; RECOVERIN; CALCIUM; BOVINE RETINA ROD CELL;

D O I：

10.1016/0014-5793(94)01030-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Rhodopsin phosphorylation in the reconstituted system consisting of urea-washed photoreceptor membranes, rhodopsin kinase and recoverin is regulated by Ca2+: the process takes place at low [Ca2+] but is suppressed at high [Ca2+]. In the absence of recoverin, rhodopsin kinase is active irrespective of the cation concentration used. Hence, recoverin is an inhibitor (at high [Ca2+]) but not an activator of rhodopsin kinase. Based jointly on these data obtained on the reconstituted system and on our preceding experiments on rod outer segments suspension, one may conclude that (i) the function of recoverin in retina rod cells is the Ca2+-sensitive control of rhodopsin phosphorylation and (ii) the presence of recoverin is essential and sufficient to provide rhodopsin kinase with the Ca2+ sensitivity.

引用

页码：171 / 172

页数：2

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