AN AMPLIFIED DNA ELEMENT IN LEISHMANIA ENCODES POTENTIAL INTEGRAL MEMBRANE AND NUCLEOTIDE-BINDING PROTEINS

被引：28

作者：

MYLER, PJ

LODES, MJ

MERLIN, G

DEVOS, T

STUART, KD

机构：

[1] SEATTLE BIOMED RES INST,SEATTLE,WA 98109

[2] UNIV WASHINGTON,DEPT PATHOBIOL,SEATTLE,WA 98195

来源：

MOLECULAR AND BIOCHEMICAL PARASITOLOGY | 1994年 / 66卷 / 01期

关键词：

LEISHMANIA; INTEGRAL MEMBRANE PROTEIN; NUCLEOTIDE-BINDING PROTEIN; LD1;

D O I：

10.1016/0166-6851(94)90031-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

LD1 is a 27.5-kb sequence that occurs in an approx. 2.2-Mb chromosome in all species and strains of Leishmania. In Leishmania infantum MHOM/BL/67/ITMAP263, LD1 is also present as an inverted dimeric repeat in multicopy, 55-kb circular molecules. Sequence analysis of a 7873-nt segment derived from the circular DNA reveals 4 open reading frames (ORFs) with potential protein coding function. One ORF predicts a protein with an ATP/GTP binding site motif. Another ORF predicts a protein with 10-12 potential membrane-spanning domains, suggesting that it encodes an integral membrane protein. This protein also has homology with that predicted by the ESAG10 gene of Trypanosoma brucei.

引用

页码：11 / 20

页数：10

共 38 条

[11]

ELLENBERGER TE, 1989, J BIOL CHEM, V264, P15094

[12] A NEW VSG EXPRESSION SITE-ASSOCIATED GENE (ESAG) IN THE PROMOTER REGION OF TRYPANOSOMA-BRUCEI ENCODES A PROTEIN WITH 10 POTENTIAL TRANSMEMBRANE DOMAINS [J].