BETA-NAP, A CEREBELLAR DEGENERATION ANTIGEN, IS A NEURON-SPECIFIC VESICLE COAT PROTEIN

We have identified a target antigen in autoimmune cerebellar degeneration, beta-NAP, that is closely related to the beta-adaptin and beta-COP coat proteins. beta-NAP is a non-clathrin-associated phosphoprotein expressed exclusively in neurons, from E12 through adulthood. beta-NAP is present in the neuronal soma and nerve terminal as soluble and membrane-bound pools and is associated with a discrete set of nerve-terminal vesicles. These results establish beta-NAP as a neuron-specific vesicle coat protein. We propose a model in which beta-NAP mediates vesicle transport between the soma and the axon terminus and suggest that beta-NAP may represent a general class of coat proteins that mediates apical transport in polarized cells.