THE MITOCHONDRIAL PYRUVATE-DEHYDROGENASE COMPLEX - NUCLEOTIDE AND DEDUCED AMINO-ACID-SEQUENCES OF A CDNA-ENCODING THE ARABIDOPSIS-THALIANA E1-ALPHA-SUBUNIT

被引：33

作者：

LUETHY, MH ^{[1
]}

MIERNYK, JA ^{[1
]}

RANDALL, DD ^{[1
]}

机构：

[1] UNIV MISSOURI, DEPT BIOCHEM, COLUMBIA, MO 65211 USA

来源：

GENE | 1995年 / 164卷 / 02期

基金：

美国国家科学基金会;

关键词：

CARBON METABOLISM; PHOSPHORYLATION; PLANT;

D O I：

10.1016/0378-1119(95)00465-I

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

A cDNA encoding the E1 alpha subunit of the Arabidopsis thaliana (At) mitochondrial (mt) pyruvate dehydrogenase complex (PDC) was sequenced. The 1435-bp cDNA consists of a 1167-bp open reading frame encoding a 43.0-kDa polypeptide of 389 amino acids (aa) (pI 7.1). The plant E1 alpha subunit has 47-51% aa sequence identity with other eukaryotic sequences. Among the regions that are highly conserved are the aa surrounding phosphorylation sites 1 and 2 of the mammalian sequence, including the conserved Ser(292) residue of At at site 1. An essential active site residue, Cys(62) Of the bovine subunit, is also conserved. A 32-aa presumptive mt targeting sequence is present at the N terminus.

引用

收藏

页码：251 / 254

页数：4

相关论文

共 21 条

[1]

ALI MS, 1993, J BIOL CHEM, V268, P22353

[2] NUCLEOTIDE AND DEDUCED AMINO-ACID SEQUENCE OF THE ALPHA-SUBUNIT OF YEAST PYRUVATE-DEHYDROGENASE [J].

BEHAL, RH ;

BROWNING, KS ;

REED, LJ .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1989, 164 (02) :941-946

[3] ACETYL-COENZYME-A CAN REGULATE ACTIVITY OF THE MITOCHONDRIAL PYRUVATE-DEHYDROGENASE COMPLEX INSITU [J].

BUDDE, RJA ;

FANG, TK ;

RANDALL, DD ;

MIERNYK, JA .

PLANT PHYSIOLOGY, 1991, 95 (01) :131-136

[4] PURIFICATION AND CHARACTERIZATION OF THE PEA CHLOROPLAST PYRUVATE-DEHYDROGENASE COMPLEX - A SOURCE OF ACETYL-COA AND NADH FOR FATTY-ACID BIOSYNTHESIS [J].

CAMP, PJ ;

RANDALL, DD .

PLANT PHYSIOLOGY, 1985, 77 (03) :571-577

[5] ISOLATION OF AN ARABIDOPSIS-THALIANA CASEIN KINASE-II BETA-SUBUNIT BY COMPLEMENTATION IN SACCHAROMYCES-CEREVISIAE [J].

COLLINGE, MA ;

WALKER, JC .

PLANT MOLECULAR BIOLOGY, 1994, 25 (04) :649-658

[6] CLONING AND SEQUENCE-ANALYSIS OF THE GENES ENCODING THE ALPHA-SUBUNIT AND BETA-SUBUNIT OF THE E1 COMPONENT OF THE PYRUVATE-DEHYDROGENASE MULTIENZYME COMPLEX OF BACILLUS-STEAROTHERMOPHILUS [J].

HAWKINS, CF ;

BORGES, A ;

PERHAM, RN .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1990, 191 (02) :337-346

[7] CHARACTERIZATION OF CDNAS ENCODING HUMAN PYRUVATE-DEHYDROGENASE ALPHA-SUBUNIT [J].

HO, L ;

WEXLER, ID ;

LIU, TC ;

THEKKUMKARA, TJ ;

PATEL, MS .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (14) :5330-5334

[8] CHARACTERIZATION OF CDNA CLONES FOR THE ALPHA SUBUNIT OF PYRUVATE-DEHYDROGENASE FROM ASCARIS-SUUM [J].

JOHNSON, KR ;

KOMUNIECKI, R ;

SUN, YH ;

WHEELOCK, MJ .

MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1992, 51 (01) :37-48

[9] ALPHA-KETO ACID DEHYDROGENASE COMPLEXES .11. COMPARATIVE STUDIES OF REGULATORY PROPERTIES OF PYRUVATE DEHYDROGENASE COMPLEXES FROM KIDNEY, HEART, AND LIVER MITOCHONDRIA [J].

LINN, TC ;

PETTIT, FH ;

HUCHO, F ;

REED, LJ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1969, 64 (01) :227-&

[10] THE NUCLEOTIDE AND DEDUCED AMINO-ACID-SEQUENCES OF A CDNA-ENCODING THE E1-BETA-SUBUNIT OF THE ARABIDOPSIS-THALIANA MITOCHONDRIAL PYRUVATE-DEHYDROGENASE COMPLEX [J].

LUETHY, MH ;

MIERNYK, JA ;

RANDALL, DD .

BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS, 1994, 1187 (01) :95-98