THE USE OF AN ALTERED SPECIFICITY ENGINEERED ENZYME FOR ASYMMETRIC-SYNTHESIS - ENANTIOSELECTIVE REDUCTION OF 4-METHYL-2-OXOPENT-3-ENOIC ACID

被引：9

作者：

CASY, G

LEE, TV

LOVELL, H

NICHOLS, BJ

SESSIONS, RB

HOLBROOK, JJ

机构：

[1] UNIV BRISTOL,DEPT CHEM,BRISTOL BS8 1TS,AVON,ENGLAND

[2] UNIV BRISTOL,DEPT BIOCHEM,BRISTOL BS8 1TS,AVON,ENGLAND

来源：

JOURNAL OF THE CHEMICAL SOCIETY-CHEMICAL COMMUNICATIONS | 1992年 / 13期

关键词：

D O I：

10.1039/c39920000924

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A genetically engineered version of Bacillus stearothermophilus lactate dehydrogenase, incorporating structural motifs which serve to alter substrate specificity in favour of alpha-keto acids with bulky aliphatic side chains, was used to effect enantioselective reduction of 4-methyl-2-oxopent-3-enoic acid.

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收藏

页码：924 / 926

页数：3

相关论文

共 14 条

[1] ALTERATION OF THE SPECIFICITY OF SUBTILISIN BPN' BY SITE-DIRECTED MUTAGENESIS IN ITS S1 AND S1' BINDING-SITES [J].

BONNEAU, PR ;

GRAYCAR, TP ;

ESTELL, DA ;

JONES, JB .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1991, 113 (03) :1026-1030

[2] ENZYMES IN ORGANIC-SYNTHESIS .45. AN EVALUATION OF THE SUBSTRATE-SPECIFICITY AND ASYMMETRIC-SYNTHESIS POTENTIAL OF THE CLONED L-LACTATE DEHYDROGENASE FROM BACILLUS-STEAROTHERMOPHILUS [J].

BUR, D ;

LUYTEN, MA ;

WYNN, H ;

PROVENCHER, LR ;

JONES, JB ;

GOLD, M ;

FRIESEN, JD ;

CLARKE, AR ;

HOLBROOK, JJ .

CANADIAN JOURNAL OF CHEMISTRY-REVUE CANADIENNE DE CHIMIE, 1989, 67 (06) :1065-1070

[3] ENANTIOSELECTIVE REDUCTION OF BETA,GAMMA-UNSATURATED ALPHA-KETO ACIDS USING BACILLUS-STEAROTHERMOPHILUS LACTATE-DEHYDROGENASE - A NEW ROUTE TO FUNCTIONALIZED ALLYLIC ALCOHOLS [J].

CASY, G ;

LEE, TV ;

LOVELL, H .

TETRAHEDRON LETTERS, 1992, 33 (06) :817-820

[4] FROM ANALYSIS TO SYNTHESIS - NEW LIGAND-BINDING SITES ON THE LACTATE-DEHYDROGENASE FRAMEWORK .2. [J].

CLARKE, AR ;

ATKINSON, T ;

HOLBROOK, JJ .

TRENDS IN BIOCHEMICAL SCIENCES, 1989, 14 (04) :145-148

[5] FROM ANALYSIS TO SYNTHESIS - NEW LIGAND-BINDING SITES ON THE LACTATE-DEHYDROGENASE FRAMEWORK .1. [J].

CLARKE, AR ;

ATKINSON, T ;

HOLBROOK, JJ .

TRENDS IN BIOCHEMICAL SCIENCES, 1989, 14 (03) :101-105

[6] ALPHA-METHOXY-ALPHA-TRIFLUOROMETHYLPHENYLACETIC ACID, A VERSATILE REAGENT FOR DETERMINATION OF ENANTIOMERIC COMPOSITION OF ALCOHOLS AND AMINES [J].

DALE, JA ;

DULL, DL ;

MOSHER, HS .

JOURNAL OF ORGANIC CHEMISTRY, 1969, 34 (09) :2543-&

[7]

FERSHT A, 1984, ENZYME MECHANISM STR, pCH14

[8]

LUBOCHIN.J, 1966, CR ACAD SCI C CHIM, V263, P732

[9]

RAMBAUD M, 1988, SYNTHESIS-STUTTGART, P564

[10]

SHAKED Z, 1980, J AM CHEM SOC, V102, P7105