PHASE-BEHAVIOR OF HETEROACID PHOSPHATIDYLSERINES AND CHOLESTEROL

被引:41
作者
BACH, D
WACHTEL, E
BOROCHOV, N
SENISTERRA, G
EPAND, RM
机构
[1] MCMASTER UNIV,HLTH SCI CTR,DEPT BIOCHEM,1200 MAIN ST W,HAMILTON L8N 3Z5,ONTARIO,CANADA
[2] WEIZMANN INST SCI,DEPT MEMBRANE RES & BIOPHYS,IL-76100 REHOVOT,ISRAEL
[3] WEIZMANN INST SCI,FAC CHEM,IL-76100 REHOVOT,ISRAEL
基金
英国医学研究理事会;
关键词
PHOSPHATIDYLSERINE; CHOLESTEROL; PHASE BEHAVIOR; DIFFERENTIAL SCANNING CALORIMETRY (DSC); X-RAY DIFFRACTION;
D O I
10.1016/0009-3084(92)90027-M
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The phase behavior of mixtures of cholesterol with 1-palmitoyl-2-oleoyl phosphatidylserine or with 1-stearoyl-2-oleoyl phosphatidylserine has been examined using differential scanning calorimetry and X-ray diffraction. We found that the miscibility of cholesterol in the bilayer is strongly affected by the small difference in length between the fully saturated fatty acid chains of the two phospholipids, Cholesterol crystallites are detected in the 1-palmitoyl-2-oleoyl-phosphatidylserine/cholesterol mixtures at a mol fraction of cholesterol of approximately 0.36, whereas for the mixtures with I-stearoyl-2-oleoyl-phosphatidylserine they appear at a cholesterol mol fraction of approximately 0.2. A phase diagram, based on differential scanning calorimetry experiments without cryoprotectant, is presented for the 1-stearoyl-2-oleoyl phosphatidylserine/cholesterol system. This phase diagram indicates the marked immiscibility of cholesterol with either the gel or the liquid crystalline phase of this phospholipid.
引用
收藏
页码:105 / 113
页数:9
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