ANALYSIS OF TRACE LEVELS OF TRICHOTHECENE MYCOTOXINS IN AUSTRIAN CEREALS BY GAS-CHROMATOGRAPHY WITH ELECTRON-CAPTURE DETECTION

被引：20

作者：

SEIDEL, V ^{[1
]}

LANG, B ^{[1
]}

FRAISSLER, S ^{[1
]}

LANG, C ^{[1
]}

SCHILLER, K ^{[1
]}

FILEK, G ^{[1
]}

LINDNER, W ^{[1
]}

机构：

[1] GRAZ UNIV,INST PHARMACEUT CHEM,A-8010 GRAZ,AUSTRIA

来源：

CHROMATOGRAPHIA | 1993年 / 37卷 / 3-4期

关键词：

GAS CHROMATOGRAPHY; ELECTRON CAPTURE DETECTION; TRICHOTHECENES; CEREAL GRAIN;

D O I：

10.1007/BF02275860

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Various analytical methods developed for trichothecene determination, including TLC, HPLC, GC, supercritical fluid chromatography (SFC) and enzyme immuno assay (EIA) are reviewed. In addition a new method is described for the simultaneous determination of the trichothecene mycotoxins deoxynivalenol (DON), nivalenol (NIV), 3-acetyldeoxynivalenol (3-ADON), diacetoxyscirpenol (DAS), T-2 toxin (T-2), HT-2 toxin (HT-2) and T-2 triol (TRIOL), in Austrian wheat and corn samples by GC-ECD. A clean-up procedure has been developed using a combination of liquid-liquid and liquid-solid extraction. Trichothecenes were detected as their heptafluorobuturyl esters or alternatively as trimethylsilyl ethers (only sensitive for deoxynivalenol and nivalenol) using nandrolone or chloramphenicol as internal standard. Four derivatization techniques using HFBI, HFBA + DMAP on polystyrene, TMSI and TMSI + BSA + TMCS have been studied and the advantages and disadvantages of each are discussed. Quantification of trichothecenes from 10 to 1000 ppb in cereals could be accomplished routinely.

引用

页码：191 / 201

页数：11

共 88 条

[1] ANTHRACENE-9-CARBONYL CHLORIDE AS A FLUORESCENCE AND ULTRAVIOLET DERIVATIZING REAGENT FOR THE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC ANALYSIS OF HYDROXY COMPOUNDS [J].