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DYSTROPHIN IN CENTRAL-NERVOUS-SYSTEM - A DEVELOPMENTAL, REGIONAL DISTRIBUTION AND SUBCELLULAR-LOCALIZATION STUDY

被引：41
作者：
JUNG, D
PONS, F
LEGER, JJ
AUNIS, D
RENDON, A
机构：
[1] CTR NEUROCHIM,INSERM,U338,5 RUE BLAISE PASCAL,F-67084 STRASBOURG,FRANCE
[2] FAC PHARM MONTPELLIER,INSERM,U300,F-34060 MONTPELLIER,FRANCE
[3] FAC PHARM MONTPELLIER,INST PATHOL GEN,F-34060 MONTPELLIER,FRANCE
来源：
NEUROSCIENCE LETTERS | 1991年 / 124卷 / 01期
关键词：
DYSTROPHIN; RAT BRAIN; MDX MOUSE; DEVELOPMENT; SUBCELLULAR LOCALIZATION; SYNAPTIC PLASMA MEMBRANE;
D O I：
10.1016/0304-3940(91)90828-H
中图分类号：
Q189 [神经科学];
学科分类号：
071006 ;
摘要：
Dystrophin, the protein encoded by the Duchenne muscular dystrophy gene has been shown to be expressed in central nervous system. In the present study, polyclonal antibodies raised against 3 fusion proteins constructed from different structural domains of dystrophin were used to identify dystrophin in protein extracts from rat and mdx mouse brain. The developmental expression of the protein, its regional distribution in rat brain and its localization in rat brain subcellular fractions were also examined. We found that dystrophin or a 'dystrophin-related protein' is expressed in mdx mouse brain. Dystrophin is detectable at very early stages of rat brain development and is expressed in all adult brain regions examined, although quantitative regional differences were found. Subcellular distribution analysis indicates that dystrophin is absent in mitochondrial and synaptic vesicle-enriched fractions but is recovered in the synaptic plasma membrane fraction.
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页码：87 / 91
页数：5
相关论文
共 30 条
[21]   THE CHICKEN DYSTROPHIN CDNA - STRIKING CONSERVATION OF THE C-TERMINAL CODING AND 3' UNTRANSLATED REGIONS BETWEEN MAN AND CHICKEN [J].
LEMAIRE, C ;
HEILIG, R ;
MANDEL, JL .
EMBO JOURNAL, 1988, 7 (13) :4157-4162
[22]   AN AUTOSOMAL TRANSCRIPT IN SKELETAL-MUSCLE WITH HOMOLOGY TO DYSTROPHIN [J].
LOVE, DR ;
HILL, DF ;
DICKSON, G ;
SPURR, NK ;
BYTH, BC ;
MARSDEN, RF ;
WALSH, FS ;
EDWARDS, YH ;
DAVIES, KE .
NATURE, 1989, 339 (6219) :55-58
[23]   IMMUNOHISTOCHEMICAL DYSTROPHIN REACTION IN SYNAPTIC REGIONS [J].
MIIKE, T ;
MIYATAKE, M ;
ZHAO, JE ;
YOSHIOKA, K ;
UCHINO, M .
BRAIN & DEVELOPMENT, 1989, 11 (05) :344-346
[24]   ISOLATION OF CANDIDATE CDNAS FOR PORTIONS OF THE DUCHENNE MUSCULAR-DYSTROPHY GENE [J].
MONACO, AP ;
NEVE, RL ;
COLLETTIFEENER, C ;
BERTELSON, CJ ;
KURNIT, DM ;
KUNKEL, LM .
NATURE, 1986, 323 (6089) :646-650
[25]   EXPRESSION OF THE PUTATIVE DUCHENNE MUSCULAR-DYSTROPHY GENE IN DIFFERENTIATED MYOGENIC CELL-CULTURES AND IN THE BRAIN [J].
NUDEL, U ;
ROBZYK, K ;
YAFFE, D .
NATURE, 1988, 331 (6157) :635-638
[26]   PURIFICATION OF NON-SYNAPTIC AND SYNAPTIC MITOCHONDRIA AND PLASMA-MEMBRANES FROM RAT-BRAIN BY A RAPID PERCOLL GRADIENT PROCEDURE [J].
RENDON, A ;
MASMOUDI, A .
JOURNAL OF NEUROSCIENCE METHODS, 1985, 14 (01) :41-51
[27]   ERYTHROCYTE SPECTRIN IS COMPRISED OF MANY HOMOLOGOUS TRIPLE HELICAL SEGMENTS [J].
SPEICHER, DW ;
MARCHESI, VT .
NATURE, 1984, 311 (5982) :177-180
[28]  
TOWBIN H, 1979, P NATL ACAD SCI USA, V70, P765
[29]   IMMUNOELECTRON MICROSCOPIC LOCALIZATION OF DYSTROPHIN IN MYOFIBERS [J].
WATKINS, SC ;
HOFFMAN, EP ;
SLAYTER, HS ;
KUNKEL, LM .
NATURE, 1988, 333 (6176) :863-866
[30]   THE DUCHENNE MUSCULAR-DYSTROPHY GENE-PRODUCT IS LOCALIZED IN SARCOLEMMA OF HUMAN SKELETAL-MUSCLE [J].
ZUBRZYCKAGAARN, EF ;
BULMAN, DE ;
KARPATI, G ;
BURGHES, AHM ;
BELFALL, B ;
KLAMUT, HJ ;
TALBOT, J ;
HODGES, RS ;
RAY, PN ;
WORTON, RG .
NATURE, 1988, 333 (6172) :466-469
123