CRYSTALLIZATION AND PRELIMINARY-X-RAY CRYSTALLOGRAPHIC STUDIES OF NATIVE ELASTASE FROM NORTH-ATLANTIC SALMON (SALMO-SALAR)

Crystals of elastase from North Atlantic salmon have been grown from 2-methyl-2,4-pentanediol by the hanging-drop vapour-diffusion method at room temperature. They grow to dimensions of 0.7 x 0.4 x 0.3 mm in three weeks. The crystals belong to the tetragonal space group P4(1)2(1)2 or P4(3)2(1)2 with cell dimensions a = b = 68.0 Angstrom and c = 84.0 Angstrom. There are eight molecules in the unit cell. The crystals diffract to at least 1.6 Angstrom, resolution and are suitable for a high-resolution crystal structure determination.