THE ADSORPTION OF BOVINE SERUM-ALBUMIN ON POSITIVELY AND NEGATIVELY CHARGED POLYSTYRENE LATTICES

被引：146

作者：

ELGERSMA, AV ^{[1
]}

ZSOM, RLJ ^{[1
]}

NORDE, W ^{[1
]}

LYKLEMA, J ^{[1
]}

机构：

[1] AKZO,CORP RES,6800 SB ARNHEM,NETHERLANDS

来源：

JOURNAL OF COLLOID AND INTERFACE SCIENCE | 1990年 / 138卷 / 01期

关键词：

D O I：

10.1016/0021-9797(90)90190-Y

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

The present study deals with the electrostatic aspects of the interaction between monomeric bovine serum albumin (BSA) and polystyrene latex particles. Adsorption and electrophoresis experiments have been performed as a function of pH, using both negatively and positively charged latices with different surface charge densities. The adsorption isotherms develop well-defined plateaus. These plateaus vary with pH, showing a maximum at the isoelectric point of the protein-covered polystyrene particles, rather than that of the isoelectric point of the protein itself. Co-adsorption of low-molecular-weight ions is shown to be an important parallel process. Studying it helps to discriminate between electrical and chemical contributions to the binding affinity. © 1990.

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页码：145 / 156

页数：12

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