POPULATION-STRUCTURE OF ACTINOBACILLUS-ACTINOMYCETEMCOMITANS - A FRAMEWORK FOR STUDIES OF DISEASE-ASSOCIATED PROPERTIES

被引：100

作者：

POULSEN, K

THEILADE, E

LALLY, ET

DEMUTH, DR

KILIAN, M

机构：

[1] AARHUS UNIV,INST MED MICROBIOL,DK-8000 AARHUS C,DENMARK

[2] AARHUS UNIV,DEPT ORAL BIOL,DK-8000 AARHUS C,DENMARK

[3] UNIV PENN,SCH DENT MED,DEPT PATHOL,PHILADELPHIA,PA 19104

[4] UNIV PENN,SCH DENT MED,DEPT BIOCHEM,PHILADELPHIA,PA 19104

来源：

MICROBIOLOGY-UK | 1994年 / 140卷

关键词：

ACTINOBACILLUS ACTINOMYCETEMCOMITANS; POPULATION GENETIC ANALYSIS; MULTILOCUS ENZYME ELECTROPHORESIS; LEUKOTOXIN GENE OPERON; RESTRICTION FRAGMENT LENGTH POLYMORPHISM;

D O I：

10.1099/13500872-140-8-2049

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The Actinobacillus actinomycetemcomitans population consists of a large number of clones among which the ubiquitous leukotoxin gene operon appears very homogeneous. Population genetic analyses performed by multilocus enzyme electrophoresis together with DNA fingerprinting and analyses of genomic DNA restriction fragment length polymorphisms (RFLP) on 97 strains isolated over a period of 45 years revealed that each of the serotypes a, b, c, d and e comprise genetically isolated subpopulations and that successful horizontal transfer of genomic DNA between strains of different serotypes appears to be extremely rare in vivo. In contrast, recombination between strains of the same serotype in general appears to take place in nature. The results provide evidence that non-serotypeable strains are serotype antigen-deficient variants originating from strains of the known serotypes. Serotype b and c strains may contain transmittable DNA sequences not found in strains of the other serotypes.

引用

页码：2049 / 2060

页数：12

共 43 条

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