FORMATION OF MULTIPLY-CHARGED IONS FROM LARGE MOLECULES USING MASSIVE-CLUSTER IMPACT

被引：40

作者：

MAHONEY, JF ^{[1
]}

CORNETT, DS ^{[1
]}

LEE, TD ^{[1
]}

机构：

[1] CITY HOPE NATL MED CTR,BECKMAN RES INT,DEPT IMMUNOL,DUARTE,CA 91010

来源：

RAPID COMMUNICATIONS IN MASS SPECTROMETRY | 1994年 / 8卷 / 05期

关键词：

D O I：

10.1002/rcm.1290080513

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Massive-cluster impact is demonstrated to be an effective ionization technique for the mass analysis of proteins as large as 17 kDa. The design of the cluster source permits coupling to both magnetic-sector and quadrupole mass spectrometers. Mass spectra are characterized by the almost total absence of chemical background and a predominance of multiply charged ions formed from 100% glycerol matrix. The number of charge states produced by the technique is observed to range from +3 to +9 for chicken egg lysozyme (14 310 Da). The lower m/z values provided by higher charge states increase the effective mass range of analyses performed with conventional ionization by fast-atom bombardment or liquid secondary ion mass spectrometry.

引用

页码：403 / 406

页数：4

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