HIGH-PERFORMANCE ANION-EXCHANGE CHROMATOGRAPHY THERMOSPRAY MASS-SPECTROMETRY IN THE ANALYSIS OF OLIGOSACCHARIDES

被引：23

作者：

NIESSEN, WMA

VANDERHOEVEN, RAM

VANDERGREEF, J

SCHOLS, HA

LUCASLOKHORST, G

VORAGEN, AGJ

BRUGGINK, C

机构：

[1] WAGENINGEN UNIV AGR,DEPT FOOD SCI,6703 HD WAGENINGEN,NETHERLANDS

[2] DIONEX BV,4801 LH BREDA,NETHERLANDS

来源：

RAPID COMMUNICATIONS IN MASS SPECTROMETRY | 1992年 / 6卷 / 07期

关键词：

D O I：

10.1002/rcm.1290060714

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The on-line coupling of high-performance anion-exchange chromatography and thermospray mass spectrometry is described. The system is applied to the analysis of neutral and acidic oligosaccharides obtained by (enzymic) degradation of plant-cell-wall polysaccharides. The features, potential and limitations of the method are discussed and exemplified with the analysis of some glucuronoxylan and xyloglycan digests. The system allows rapid molecular-mass determination of components in oligomer mixtures with 1-10-mu-g injected amounts.

引用

收藏

页码：474 / 478

页数：5

相关论文

共 12 条

[1] COMBINED LIQUID-CHROMATOGRAPHY MASS-SPECTROMETRY .2. TECHNIQUES AND MECHANISMS OF THERMOSPRAY [J].

ARPINO, P .

MASS SPECTROMETRY REVIEWS, 1990, 9 (06) :631-669

[2] ION CHROMATOGRAPHY MASS-SPECTROMETRY FOR THE DETERMINATION OF ORGANIC AMMONIUM AND SULFATE COMPOUNDS [J].

CONBOY, JJ ;

HENION, JD ;

MARTIN, MW ;

ZWEIGENBAUM, JA .

ANALYTICAL CHEMISTRY, 1990, 62 (08) :800-807

[3]

DUSTERHOFT EM, IN PRESS J SCI FOOD

[4] HIGH-PERFORMANCE ANION-EXCHANGE CHROMATOGRAPHY FOR CARBOHYDRATE ANALYSIS [J].

LEE, YC .

ANALYTICAL BIOCHEMISTRY, 1990, 189 (02) :151-162

[5] ONLINE LIQUID-CHROMATOGRAPHY THERMOSPRAY MASS-SPECTROMETRY IN THE ANALYSIS OF OLIGOSACCHARIDES [J].

NIESSEN, WMA ;

VANDERHOEVEN, RAM ;

VANDERGREEF, J ;

SCHOLS, HA ;

VORAGEN, AGJ .

RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 1992, 6 (03) :197-202

[6] PRODUCTS OF HYDROLYSIS OF BEECHWOOD ACETYL-4-O-METHYLGLUCURONOXYLAN BY A XYLANASE AND AN ACETYL XYLAN ESTERASE [J].

PULS, J ;

TENKANEN, M ;

KORTE, HE ;

POUTANEN, K .

ENZYME AND MICROBIAL TECHNOLOGY, 1991, 13 (06) :483-486

[7] USE OF REVERSED-PHASE CHROMATOGRAPHY IN CARBOHYDRATE ANALYSIS [J].

RAJAKYLA, E .

JOURNAL OF CHROMATOGRAPHY, 1986, 353 :1-12

[8] ADAPTATION OF A THERMOSPRAY LIQUID-CHROMATOGRAPHY MASS-SPECTROMETRY INTERFACE FOR USE WITH ALKALINE ANION-EXCHANGE LIQUID-CHROMATOGRAPHY OF CARBOHYDRATES [J].

SIMPSON, RC ;

FENSELAU, CC ;

HARDY, MR ;

TOWNSEND, RR ;

LEE, YC ;

COTTER, RJ .

ANALYTICAL CHEMISTRY, 1990, 62 (03) :248-252

[9]

VENEKAMP JC, 1991, COMMUNICATION

[10] ANALYSIS OF OLIGOMERIC AND MONOMERIC SACCHARIDES FROM ENZYMATICALLY DEGRADED POLYSACCHARIDES BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].

VORAGEN, AGJ ;

SCHOLS, HA ;

SEARLEVANLEEUWEN, MF ;

BELDMAN, G ;

ROMBOUTS, FM .

JOURNAL OF CHROMATOGRAPHY, 1986, 370 (01) :113-120