PARTIAL-PURIFICATION AND SOME PROPERTIES OF ALCOHOL ACYLTRANSFERASE FROM STRAWBERRY FRUITS

被引：78

作者：

PEREZ, AG ^{[1
]}

SANZ, C ^{[1
]}

OLIAS, JM ^{[1
]}

机构：

[1] CSIC,INST GRASA & DERIVADOS,UEI FISIOL & TECNOL POST RECOLECC,APDO 1078,E-41012 SEVILLE,SPAIN

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 1993年 / 41卷 / 09期

关键词：

D O I：

10.1021/jf00033a021

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

The enzyme system concerning volatile ester formation in strawberry Fragaria ananassa x Duchessne var. Chandler was studied. Protein with alcohol acyltransferase activity was purified about 29-fold from Chandler strawberry fruits by ammonium sulfate fractionation, gel filtration, and anion-exchange chromatography. The enzyme activity had a pH optimum of 8.0 and an optimum temperature of 35-degrees-C. The apparent M(r) estimated by gel filtration was 70 000. The enzyme was tested for its preference in using different acyl-CoAs and alcohols. Maximum activity was obtained using acetyl-CoA and hexyl alcohol as substrates. A clear correlation was observed between substrate preference and volatile esters present in strawberry var. Chandler.

引用

页码：1462 / 1466

页数：5

共 26 条

[11] Nordstron K., 1961, J I BREWING, V67, P173
[12] AROMA COMPONENTS AND FREE AMINO-ACIDS IN STRAWBERRY VARIETY CHANDLER DURING RIPENING
PEREZ, AG
RIOS, JJ
SANZ, C
OLIAS, JM
[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1992, 40 (11) : 2232 - 2235
[13] PERRIN DD, 1979, BUFFER PH METAL ION, P155
[14] BIOGENESIS OF AROMA CONSTITUENTS OF FRUITS AND VEGETABLES
SALUNKHE, DK
DO, JY
[J]. CRC CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION, 1976, 8 (02): : 161 - 190
[15] PRODUCTION OF ODORIFEROUS-GAMMA-LACTONES BY FUSARIUM-POAE
SARRIS, J
LATRASSE, A
[J]. AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1985, 49 (11): : 3227 - 3230
[16] BIOGENESIS OF BANANA VOLATILES
TRESSL, R
DRAWERT, F
[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1973, 21 (04) : 560 - 565
[17] UEDA Y, 1978, NIPPON SHOKUHIN KOGY, V25, P440
[18] Ueda Y., 1977, NIPPON SHOKUHIN KOGY, V24, P624, DOI DOI 10.3136/NSKKK1962.24.624
[19] UEDA Y, 1976, NIPPON SHOKUHIN KOGY, V23, P288
[20] PROPERTIES OF DIACYLGLYCEROL ACYLTRANSFERASE FROM MICROSPORE-DERIVED EMBRYOS OF BRASSICA-NAPUS
WESELAKE, RJ
TAYLOR, DC
POMEROY, MK
LAWSON, SL
UNDERHILL, EW
[J]. PHYTOCHEMISTRY, 1991, 30 (11) : 3533 - 3538

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