A NOVEL PROTEIN ASSAY-METHOD USING TETRAPHENYLPORPHIN TETRASULFONATE (TPPS4)

被引：41

作者：

LI, N

LI, KA

TONG, SY

机构：

[1] Department of Chemistry, Peking University, Beijing

来源：

ANALYTICAL LETTERS | 1995年 / 28卷 / 10期

关键词：

PROTEIN ASSAY; TETRAPHENYLPORPHIN TETRASULFONATE; STAINING; SPECTROPHOTOMETRY;

D O I：

10.1080/00032719508000354

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A sensitive protein assay method which involves the reaction of TPPS4 with protein is described. When protein is added to TPPS4 solution, an absorption band with the maximum at 488 nm appears and the absorbance is proportional to the concentration of protein. Just Like the Soret absorption of the porphyrin, the new band is very narrow and there is no overlap at all between them, which means the free dyes would not give any background for the detection of the protein-TPPS4 complexes. A new spectrophotometric method for determination of protein has been constructed and applied to the determination of human plasma protein and urinary protein; The assay using microtiter plates has also been studied.

引用

页码：1763 / 1774

页数：12

共 21 条

[11]

LOTT JA, 1983, CLIN CHEM, V29, P1946

[12]

LOWRY OH, 1951, J BIOL CHEM, V193, P265

[13] AN IMPROVEMENT OF THE COOMASSIE BLUE-DYE BINDING METHOD ALLOWING AN EQUAL SENSITIVITY TO VARIOUS PROTEINS - APPLICATION TO CEREBROSPINAL-FLUID [J].

MACART, M ;

GERBAUT, L .

CLINICA CHIMICA ACTA, 1982, 122 (01) :93-101

[14]

MARSHALL T, 1987, CLIN CHEM, V33, P577

[15]

MCINTOSH JC, 1977, CLIN CHEM, V23, P1939

[16]

ORSONNEAU JL, 1989, CLIN CHEM, V35, P2233

[17] AGGREGATION OF MESO-SUBSTITUTED WATER-SOLUBLE PORPHYRINS [J].

PASTERNA.RF ;

CENTURO, GC ;

BOYD, P ;

HINDS, LD ;

HUBER, PR ;

FRANCESC.L ;

FASELLA, P ;

ENGASSER, G ;

GIBBS, E .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1972, 94 (13) :4511-+

[18]

REDINBAUGH MG, 1985, ANAL BIOCHEM, V47, P144

[19] MEASUREMENT OF PROTEIN USING BICINCHONINIC ACID [J].

SMITH, PK ;

KROHN, RI ;

HERMANSON, GT ;

MALLIA, AK ;

GARTNER, FH ;

PROVENZANO, MD ;

FUJIMOTO, EK ;

GOEKE, NM ;

OLSON, BJ ;

KLENK, DC .

ANALYTICAL BIOCHEMISTRY, 1985, 150 (01) :76-85

[20] ELECTROPHORETIC SEPARATION OF LARGE PROTEOGLYCANS IN LARGE-PORE POLYACRYLAMIDE GRADIENT GELS (1.32-10.0-PERCENT-T) AND A ONE-STEP PROCEDURE FOR SIMULTANEOUS STAINING OF PROTEINS AND PROTEOGLYCANS [J].

VILIM, V ;

KRAJICKOVA, J .

ANALYTICAL BIOCHEMISTRY, 1991, 197 (01) :34-39

← 1 2 3 →