TRANSPORT OF 4-HYDROXYPHENYLACETIC ACID IN KLEBSIELLA-PNEUMONIAE

被引：22

作者：

ALLENDE, JL

GIBELLO, A

MARTIN, M

GARRIDOPERTIERRA, A

机构：

[1] UNIV COMPLUTENSE MADRID, FAC VET, DEPT BIOQUIM & BIOL MOLEC 4, AVENIDA PUERTA HIERRO S-N, E-28040 MADRID, SPAIN

[2] UNIV COMPLUTENSE MADRID, FAC VET, DEPT FISIOL, E-28040 MADRID, SPAIN

[3] UNIV COMPLUTENSE MADRID, FAC VET, DEPT MICROBIOL PATOL ANIM, E-28040 MADRID, SPAIN

来源：

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS | 1992年 / 292卷 / 02期

关键词：

D O I：

10.1016/0003-9861(92)90034-T

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Klebsiella pneumoniae M5a1 has been shown to possess an inducible transport system for 4-hydroxyphenylacetate (4-HPA). This transport system has a Kt of 16.3 μm and a maximal velocity of 31.2 nmol/min (milligrams dry weight). The transport system has been inhibited by inhibitors of energy metabolism with a concomitant decrease in cellular ATP concentrations, and the 4-HPA binding activity has been detected in the crude shock extracts. All these observations indicate that 4-HPA uptake is an active transport which involves a periplasmic binding protein and it seems to be energized by phosphate bond energy. © 1992.

引用

页码：583 / 588

页数：6

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