GLUTAMATE OXIDASE ENZYME ELECTRODES - MICROSENSORS FOR NEUROTRANSMITTER DETERMINATION USING ELECTROCHEMICALLY POLYMERIZED PERMSELECTIVE FILMS

被引：71

作者：

COOPER, JM

FOREMAN, PL

GLIDLE, A

LING, TW

PRITCHARD, DJ

机构：

[1] Department of Electronics and Electrical Engineering, University of Glasgow, Glasgow

来源：

JOURNAL OF ELECTROANALYTICAL CHEMISTRY | 1995年 / 388卷 / 1-2期

关键词：

ENZYME MICROSENSORS; ELECTROPOLYMERIZATION; NEUROTRANSMITTERS; GLUTAMATE OXIDASE; PERMSELECTIVE FILMS;

D O I：

10.1016/0022-0728(95)03846-9

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Enzyme entrapment in electrochemically polymerized membranes is an attractive method for the development of stable reproducible enzyme microsensors with fast response times. In this paper the development of a biosensor for the determination of glutamate, involving the entrapment of the enzyme glutamate oxidase in two different electropolymerization matrices, is described. Subsequently, the kinetics of the immobilized enzyme in the different matrices are compared, and the role of the polymers in providing a permselective barrier for the exclusion of potential electroactive interferents are characterized.

引用

页码：143 / 149

页数：7

共 20 条

[1] ELECTROCHEMICAL IMMOBILIZATION OF ENZYMES .1. THEORY [J].