THE MATURE FORM OF IMPORTED MITOCHONDRIAL PROTEINS UNDERGOES CONFORMATIONAL-CHANGES UPON BINDING TO ISOLATED-MITOCHONDRIA

被引:24
作者
HARTMANN, CM [1 ]
GEHRING, H [1 ]
CHRISTEN, P [1 ]
机构
[1] UNIV ZURICH,INST BIOCHEM,WINTERTHURERSTR 190,CH-8057 ZURICH,SWITZERLAND
来源
EUROPEAN JOURNAL OF BIOCHEMISTRY | 1993年 / 218卷 / 03期
关键词
D O I
10.1111/j.1432-1033.1993.tb18446.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Mature mitochondrial proteins (aspartate aminotransferase, malate dehydrogenase, hydroxyacyl coenzyme A dehydrogenase, creatine kinase) and cytosolic proteins (aldolase, glyceraldehyde-3-phosphate dehydrogenase) with a basic pI were found to bind to isolated mitochondria, electrostatic interactions being mainly responsible for their binding. Mitochondrial aspartate aminotransferase bound with a K(d), of 30 nM in 0.6 M sorbitol, 20 mM Hepes/KOH, pH 7.4, at 25-degrees-C. Cytosolic aspartate aminotransferase and glutamate dehydrogenase (a protein located in the mitochondrial matrix) both with an acidic pl, did not bind to mitochondria. Treatment of mitochondria with proteinases did not affect the subsequent binding of imported mitochondrial proteins. Their association with both intact and proteinase-treated mitochondria resulted in a marked increase in their susceptibility toward proteinase K. In contrast, the basic cytosolic proteins tested bound only to intact mitochondria and thereby did not become more susceptible toward proteolytic attack. Treatment of mitochondria with adriamycin, a drug binding to acidic phospholipids, prevented the subsequent association of mitochondrial aspartate aminotransferase with mitochondria and the ensuing conformational labilization. Apparently, the mature moiety of imported mitochondrial proteins is partially unfolded upon interaction with the lipid component of the mitochondrial envelope. Both the binding of the mitochondrial proteins and their conformational labilization is independent of ATP and the electrochemical potential across the inner membrane.
引用
收藏
页码:905 / 910
页数:6
相关论文
共 30 条
[21]   DISRUPTION OF THE OUTER-MEMBRANE RESTORES PROTEIN IMPORT TO TRYPSIN-TREATED YEAST MITOCHONDRIA [J].
OHBA, M ;
SCHATZ, G .
EMBO JOURNAL, 1987, 6 (07) :2117-2122
[22]  
OLSON JA, 1952, J BIOL CHEM, V197, P67
[23]   THE MITOCHONDRIAL PROTEIN IMPORT APPARATUS [J].
PFANNER, N ;
NEUPERT, W .
ANNUAL REVIEW OF BIOCHEMISTRY, 1990, 59 :331-353
[24]  
PFISTER K, 1985, J BIOL CHEM, V260, P1414
[25]   INTERACTION OF MITOCHONDRIAL CREATINE-KINASE WITH MODEL MEMBRANES - A MONOLAYER STUDY [J].
ROJO, M ;
HOVIUS, R ;
DEMEL, R ;
WALLIMANN, T ;
EPPENBERGER, HM ;
NICOLAY, K .
FEBS LETTERS, 1991, 281 (1-2) :123-129
[26]  
SCHATZ G, 1991, HARVEY LECT, V85, P109
[27]  
SCHLEGEL J, 1988, J BIOL CHEM, V263, P16963
[28]  
SMITH CM, 1972, J BIOL CHEM, V247, P273
[29]  
SONDEREGGER P, 1982, J BIOL CHEM, V257, P3339
[30]   A METHOD FOR THE QUANTITATIVE RECOVERY OF PROTEIN IN DILUTE-SOLUTION IN THE PRESENCE OF DETERGENTS AND LIPIDS [J].
WESSEL, D ;
FLUGGE, UI .
ANALYTICAL BIOCHEMISTRY, 1984, 138 (01) :141-143