PROPERTIES OF THE TUNGSTEN-SUBSTITUTED MOLYBDENUM FORMYLMETHANOFURAN DEHYDROGENASE FROM METHANOBACTERIUM-WOLFEI

被引：43

作者：

SCHMITZ, RA

ALBRACHT, SPJ

THAUER, RK

机构：

[1] MAX PLANCK INST TERRESTR MIKROBIOL,MARBURG,GERMANY

[2] UNIV AMSTERDAM,EC SLATER INST BIOCHEM RES,1018 NL AMSTERDAM,NETHERLANDS

来源：

FEBS LETTERS | 1992年 / 309卷 / 01期

关键词：

FORMYLMETHANOFURAN DEHYDROGENASE; TUNGSTEN-SUBSTITUTED MOLYBDENUM PROTEIN; TUNGSTEN EPR SIGNAL; METHANOGENIC ARCHAEA;

D O I：

10.1016/0014-5793(92)80743-Z

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In Methanobacterium wolfei two formylmethanofuran dehydrogenases are present. one of which is a molybdenum- and the other a tungsten enzyme. We report here that also the 'molybdenum' enzyme contained tungsten when the archaeon was grown on molybdenum-deprived medium supplemented with tungstate (1-mu-M). Unexpectedly the tungsten-substituted molybdenum enzyme was catalytically active and displayed a rhombic EPR signal which was attributed to tungsten by the characteristic W-183 splitting.

引用

页码：78 / 81

页数：4

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