AMYLIN INHIBITS GLUCOSE-INDUCED INSULIN-SECRETION IN A DOSE-DEPENDENT MANNER - STUDY IN THE PERFUSED RAT PANCREAS

被引：71

作者：

DEGANO, P ^{[1
]}

SILVESTRE, RA ^{[1
]}

SALAS, M ^{[1
]}

PEIRO, E ^{[1
]}

MARCO, J ^{[1
]}

机构：

[1] UNIV AUTONOMA MADRID,HOSP PUERTA HIERRO,SAN MARTIN PORRES 4,E-28035 MADRID,SPAIN

来源：

REGULATORY PEPTIDES | 1993年 / 43卷 / 1-2期

关键词：

RAT PANCREAS; RAT AMYLIN; INSULIN RELEASE;

D O I：

10.1016/0167-0115(93)90411-Z

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Islet amyloid polypeptide (IAPP), also called amylin, has been localized in the B-cell secretory granule and is co-secreted with insulin. We have investigated the effect of synthetic amidated rat amylin on the insulin release evoked by 9 mM glucose in the isolated, perfused rat pancreas. Amylin, in a range of 75 nM-75 pM, significantly inhibited this insulin response in a dose-dependent manner. The correlation between the logarithm of amylin concentrations and the percentages of inhibition was highly significant (r = 0.98, P< 0.01). The lowest effective amylin concentration tested (75 pM) is within the range of amylin levels reported for the effluent of the perfused rat pancreas. Finally, pre-infusion of the rat pancreas with a high amylin concentration (75 nM) did not alter the insulin response to glucose, thus excluding a toxic effect of amylin on the B-cell. These observations support the concept that amylin plays a role in the control of insulin secretion.

引用

页码：91 / 96

页数：6

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