ULTRAFAST SPECTROSCOPY OF THE VISUAL PIGMENT RHODOPSIN

被引:47
作者
YAN, M
MANOR, D
WENG, G
CHAO, H
ROTHBERG, L
JEDJU, TM
ALFANO, RR
CALLENDER, RH
机构
[1] CUNY CITY COLL, DEPT PHYS, NEW YORK, NY 10031 USA
[2] CUNY CITY COLL, DEPT ELECT ENGN, NEW YORK, NY 10031 USA
[3] CUNY CITY COLL, INST ULTRAFAST SPECT & LASERS, NEW YORK, NY 10031 USA
[4] AT&T BELL LABS, MURRAY HILL, NJ 07974 USA
关键词
BATHORHODOPSIN; SUBPICOSECOND; ABSORPTION; PRIMARY PROCESSES;
D O I
10.1073/pnas.88.21.9809
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
We report on time-resolved absorption studies of the bovine visual pigment rhodopsin with subpicosecond resolution at room temperature. Our data show that bathorhodopsin, rhodopsin's early photoproduct, is photochemically formed in 3.0 +/- 0.7 ps. The data suggest that bathorhodopsin formation is kinetically preceded by two species along the rhodopsin-to-bathorhodopsin reaction coordinate. The first is identified with the vertically excited Franck-Condon state. This decays with an almost-equal-to 200-fs lifetime to an intermediate, which then decays to bathorhodopsin in 3.0 ps. We assign this intermediate to be an excited state transient near 90-degrees along the 11-12 torsional coordinate of rhodopsin's chromophore. Exchange of rhodopsin's exchangeable protons for deuterons does not affect the observed dynamics. These observations are both qualitatively and quantitatively consistent with molecular dynamics calculations, which model the rhodopsin to bathorhodopsin phototransition as a cis-trans isomerization along the 11-12 torsional coordinate of rhodopsin's chromophore.
引用
收藏
页码:9809 / 9812
页数:4
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