RAPID HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC METHOD FOR THE QUANTITATION OF POLYAMINES AS THEIR DANSYL DERIVATIVES - APPLICATION TO PLANT AND ANIMAL-TISSUES

被引：133

作者：

MARCE, M

BROWN, DS

CAPELL, T

FIGUERAS, X

TIBURCIO, AF

机构：

[1] UNIV BARCELONA,FAC FARM,FISIOL VEGETAL LAB,E-08028 BARCELONA,SPAIN

[2] ROWETT RES INST,ABERDEEN AB2 9SB,SCOTLAND

来源：

JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS | 1995年 / 666卷 / 02期

关键词：

D O I：

10.1016/0378-4347(94)00586-T

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A rapid high-performance liquid chromatographic method for the separation of polyamines as their dansyl derivative has been developed. The chromatographic system used consisted of a reversed-phase column and a mobile phase of acetonitrile and water. The separation of 1,3-diaminopropane, putrescine, cadaverine, spermidine and spermine takes only 9 min. This method provides a good resolution between 1,3-diaminopropane and putrescine. It has been applied to quantify polyamines from seeds of wheat, petals of Phalaenopsis hybrids and various rat tissues.

引用

收藏

页码：329 / 335

页数：7

相关论文

共 18 条

[1] IDENTIFICATION OF CADAVERINE IN PISUM-SATIVUM [J].

ANDERSON, JN ;

MARTIN, RO .

PHYTOCHEMISTRY, 1973, 12 (02) :443-446

[2] AN IMPROVED METHOD FOR ANALYSIS OF POLYAMINES IN PLANT-TISSUE BY PRECOLUMN DERIVATIZATION WITH ORTHO-PHTHALALDEHYDE AND SEPARATION BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].

CORBIN, JL ;

MARSH, BH ;

PETERS, GA .

PLANT PHYSIOLOGY, 1989, 90 (02) :434-439

[3] ANALYSIS OF POLYAMINES IN HIGHER-PLANTS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].

FLORES, HE ;

GALSTON, AW .

PLANT PHYSIOLOGY, 1982, 69 (03) :701-706

[4]

HAYMAN AR, 1985, J CHROMATOGR, V325, P462, DOI 10.1016/S0021-9673(00)96058-8

[5]

KABRA PM, 1986, J CHROMATOGR, V380, P19, DOI 10.1016/S0378-4347(00)83621-X

[6] ANALYSIS OF POLYAMINES AS THEIR DABSYL DERIVATIVES BY REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].

KOSKI, P ;

HELANDER, IM ;

SARVAS, M ;

VAARA, M .

ANALYTICAL BIOCHEMISTRY, 1987, 164 (01) :261-266

[7]

LIN KJ, 1982, J CHROMATOGR, V227, P369

[8] POLYAMINE DETERMINATION IN CLINICAL LABORATORIES BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].

MATSUMOTO, T ;

TSUDA, T ;

SUZUKI, O .

TRAC-TRENDS IN ANALYTICAL CHEMISTRY, 1990, 9 (09) :292-297

[9] DETERMINATION OF POLYAMINES IN RAT-TISSUES BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY AFTER DERIVATIZATION WITH 1-PHENYLSULFONYL-3,3,3-TRIFLUOROPROPENE [J].

NAKAJIMA, M ;

WAKABAYASHI, H ;

YAMATO, S ;

SHIMADA, K .

ANALYTICAL SCIENCES, 1990, 6 (04) :523-527

[10] HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF POLYAMINES IN SELECTED VEGETABLES WITH POSTCOLUMN FLUOROMETRIC DERIVATIZATION [J].

OHTA, H ;

TAKEDA, Y ;

YOZA, KI ;

NOGATA, Y .

JOURNAL OF CHROMATOGRAPHY, 1993, 628 (02) :199-204