METHODOLOGY OF GENERATION AND CHARACTERISTICS OF SIMPLE SEQUENCE REPEAT DNA MARKERS IN AVOCADO (PERSEA-AMERICANA M)

被引：45

作者：

LAVI, U ^{[1
]}

AKKAYA, M ^{[1
]}

BHAGWAT, A ^{[1
]}

LAHAV, E ^{[1
]}

CREGAN, PB ^{[1
]}

机构：

[1] USDA ARS, BELTSVILLE AGR RES CTR, SOYBEAN & ALFALFA RES LAB, BELTSVILLE, MD 20705 USA

来源：

EUPHYTICA | 1994年 / 80卷 / 03期

关键词：

BREEDING; GENETICS; MICROSATELLITES; PCR; SSR; AVOCADO; PERSEA AMERICANA;

D O I：

10.1007/BF00039648

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Generation of Simple Sequence Repeat (SSR) DNA markers was based on the construction of genomic DNA library of avocado (Persea americana M.). The library was screened with the four dinucleotide probes (AG), (AT), (GC) and (CA). Positive clones were sequenced to validate the presence of simple sequence repeats (SSR) and to generate polymerase chain reaction (PCR) primers based on the sequences flanking the simple sequence repeat. Twenty six different pairs of primers which yield a PCR product in the initial screening were synthesized. The SSR A1E11 was found to have eleven alleles while A3F8 has eight alleles. The SSRs in avocado were found to be inherited in a Mendelian fashion.

引用

页码：171 / 177

页数：7