ACETYLCHOLINESTERASE-BASED AMPEROMETRIC SENSOR

被引：23

作者：

STOYTCHEVA, M

机构：

[1] Department of Chemistry, Institute for Foreign Students, Sofia, 1113

来源：

ELECTROANALYSIS | 1995年 / 7卷 / 06期

关键词：

BIOSENSOR; ACETYLCHOLINESTERASE; ACETYLTHIOCHOLINE IODIDE;

D O I：

10.1002/elan.1140070610

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An amperometric sensor has been created using the hydrolysis of acetylthiocholine iodide, which is catalyzed by the acetylcholinesterase enzyme and generates an electroactive product. An electrochemically modified graphite electrode is used, with acetylcholinesterase covalently bonded directly to its surface. The characteristics of the sensor, such as the response time, repeatability, reproducibility, stability, dynamic range, activation energy, and temperature coefficient of the current are evaluated. The optimum working conditions (pH, temperature, and rotation speed) are established.

引用

页码：560 / 562

页数：3

共 12 条

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