DETERMINATION OF A CHOLESTEROL OXIDE MIXTURE BY A SINGLE-RUN HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC ANALYSIS USING BENZOYLATION

被引：13

作者：

FILLION, L

ZEE, JA

GOSSELIN, C

机构：

[1] UNIV LAVAL,FAC SCI & ALIMENTAT,DEPT NUTR HUMAINE & CONSOMMAT,QUEBEC CITY G1K 7P4,QUEBEC,CANADA

[2] UNIV LAVAL,FAC SCI AGR & ALIMENTAT,DEPT SCI & TECHNOL ALIMENTS,QUEBEC CITY G1K 7P4,QUEBEC,CANADA

来源：

JOURNAL OF CHROMATOGRAPHY | 1991年 / 547卷 / 1-2期

关键词：

D O I：

10.1016/S0021-9673(01)88633-7

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A rapid, single-run high-performance liquid chromatographic method has been developed to separate and quantify benzoate esters of cholesterol oxides, providing high sensitivity via ultraviolet detection. The mobile phase was 85% isopropanol - water (v/v). Analyses of 7-ketocholesterol, cholestane-triol, epoxy-cholesterol, 7-hydroxycholesterol and 25-hydroxycholesterol were done using a 30 cm x 3.9 mm I.D. Novapak C18 column and a variable-wavelength ultraviolet detector (set at 230 nm). Linearity was excellent since a good correlation was observed. As low as 500 ng of cholesterol benzoate per 20-mu-l of solution can be detected by this method.

引用

页码：105 / 112

页数：8

共 21 条

[11] DETECTION OF STEROL EPOXIDES IN FOODS BY COLORIMETRIC REACTION WITH PICRIC ACID [J].