TRANSFORMATION OF GIBBERELLA-FUJIKUROI - EFFECT OF THE ASPERGILLUS-NIDULANS AMA1 SEQUENCE ON FREQUENCY AND INTEGRATION

被引：26

作者：

BRUCKNER, B

UNKLES, S

WELTRING, K

KINGHORN, JR

机构：

[1] UNIV ST ANDREWS, PLANT MOLEC GENET UNIT, ST ANDREWS KY16 9TH, FIFE, SCOTLAND

[2] UNIV MUNSTER, INST BOT, W-4400 MUNSTER, GERMANY

来源：

CURRENT GENETICS | 1992年 / 22卷 / 04期

关键词：

FILAMENTOUS FUNGI; GENETIC TRANSFORMATION; ARGININE AUXOTROPHY; HYGROMYCIN RESISTANCE; GIBBERELLINS;

D O I：

10.1007/BF00317927

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

A stable and reproducible transformation selection system for Gibberella fujikuroi protoplasts based on the Aspergillus nidulans argB gene, encoding ornithine transcarbamylase, has been developed. Inclusion into the vector of the A. nidulans DNA fragment (AMA1), which permits plasmid autonomous replication in A. nidulans, A. niger and A. oryzae, appeared to permit autonomous replication of G. fujikuroi although the transformation frequency was increased by only two-fold. Transformation was also achieved using the bacterial hygromycin B resistance gene under the control of G. pulicaris and A. nidulans promoters.

引用

页码：313 / 316

页数：4

共 15 条

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