CHROMOSOMAL-ABERRATIONS INDUCED IN HUMAN WHOLE-BLOOD CULTURES BY PIPETTING CELL PELLETS IN THE PRESENCE OF ALUI

被引：6

作者：

LOPEZ, WM

PIEPER, R

OBE, G

机构：

[1] UNIV GH ESSEN,DEPT GENET,D-45117 ESSEN,GERMANY

[2] INST INVEST BIOL C ESTABLE,DIV HUMAN CYTOGENET & QUANTITAT MICROSCOPY,MONTEVIDEO 11600,URUGUAY

来源：

MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS | 1995年 / 327卷 / 1-2期

关键词：

HUMAN WHOLE-BLOOD CULTURE; CHROMOSOME ABERRATION INDUCTION; RESTRICTION ENDONUCLEASE;

D O I：

10.1016/0027-5107(94)00065-D

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A physical method is described to permeabilize human peripheral lymphocytes in culture for the restriction endonuclease AluI. Blood cultures are incubated for 20 h, pelleted, strongly pipetted with different types of Pasteur pipettes in the presence of AluI and recovered for up to 54 h in the presence of 5-bromodeoxyuridine. Up to 20% of aberrant first posttreatment (M1) metaphases with chromosome-type aberrations are found. The method works with whole blood cultures and is therefore easy to perform.

引用

页码：23 / 31

页数：9

共 7 条

[1] ARE ROGUE CELLS AN INDICATOR OF CANCER RISK DUE TO THE ACTION OF BACTERIAL RESTRICTION ENDONUCLEASES [J].