PROTEINACEOUS INHIBITOR FROM SOLANUM-TUBEROSUM INVERTASE

被引：12

作者：

ISLA, MI

VATTUONE, MA

SAMPIETRO, AR

机构：

[1] Instituto de Estudios Vegetales, Cåtedra de Fitoquímica, Facultad de Bioquímica, Química y Farmacia

来源：

PHYTOCHEMISTRY | 1991年 / 30卷 / 03期

关键词：

SOLANUM-TUBEROSUM; SOLANACEAE; INVERTASE; LECTIN; ACTIVATION BY PROTEIN; REGULATORY ENZYME; PROTEINACEOUS INHIBITOR;

D O I：

10.1016/0031-9422(91)85244-T

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The lectin from Solanum tuberosum is shown to be an inhibitor of invertase from the same plant. However, on the basis of pH curves and the dissociability of the respective complexes, a difference between lectin and proteinaceous inhibitors was established. This difference was confirmed by affinity chromatography on chitin columns. Kinetics of lectin inhibition is also described. Competition experiments between potato lectin and the proteinaceous inhibitor suggest that both substances are mutually exclusive. The proteinaceous inhibitor was also shown to agglutinate human erythrocytes.

引用

页码：739 / 743

页数：5

共 18 条

[1] INHIBITION OF POTATO-TUBER INVERTASE BY AN ENDOGENOUS INHIBITOR - EFFECTS OF SALTS, PH, TEMPERATURE, AND SUGARS ON BINDING [J].