HEAT-CONDUCTION CALORIMETERS - TIME CONSTANTS, SENSITIVITY AND FAST TITRATION EXPERIMENTS

被引：69

作者：

BACKMAN, P

BASTOS, M

HALLEN, D

LONNBRO, P

WADSO, I

机构：

[1] Division of Thermochemistry, Chemical Center, University of Lund, S-221 00 Lund

来源：

JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS | 1994年 / 28卷 / 02期

关键词：

HEAT CONDUCTION CALORIMETER; MICROCALORIMETER; PRECISION; SENSITIVITY; TIME CONSTANT; DYNAMIC CORRECTION; TITRATION;

D O I：

10.1016/0165-022X(94)90023-X

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Seven heat conduction calorimeters have been evaluated in terms of sensitivity and thermal response time. The use of a dynamic method to correct for the thermal inertia of the calorimeter is shown to reduce by one order of magnitude the time required to conduct a stepwise titration experiment involving a fast reaction. The ability to determine association constants of strong 1:1 complexes has been evaluated in terms of the precision of determining thermal energy.

引用

页码：85 / 100

页数：16

共 16 条

[1]

[Anonymous], 1969, DATA REDUCTION ERROR

[2] CELL-GROWTH EXPERIMENTS USING A MICROCALORIMETRIC VESSEL EQUIPPED WITH OXYGEN AND PH ELECTRODES [J].