CHARACTERIZATION OF ROSMARINIC ACID SYNTHASE FROM CELL-CULTURES OF COLEUS-BLUMEI

被引:58
作者
PETERSEN, MS
机构
[1] Institut für Entwicklungs- und Molekularbiologie der Pflanzen, Heinrich-Heine-Universität Düsseldorf, D-4000 Düsseldorf 1
关键词
COLEUS-BLUMEI; LAMIACEAE; ROSMARINIC ACID BIOSYNTHESIS; ACYLTRANSFERASE; ROSMARINIC ACID; HYDROXYCINNAMIC ACID ESTERS; HYDROXYPHENYLLACTIC ACID;
D O I
10.1016/S0031-9422(00)98217-7
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Rosmarinic acid synthase (RAS) catalyses the transesterification reaction of CoA-activated cinnamic acids, e.g. 4-coumaroyl- or caffeoyl-CoA, and hydroxyphenyllactic acids, e.g. 4-hydroxy- or 3,4-dihydroxyphenyllactic acid, during the biosynthesis of rosmarinic acid. The enzyme isolated from suspension cultures of Coleus blumei is soluble and has an optimal pH of 7.0-7.5. Michaelis-Menten kinetics were observed with respect to the substrates 4-coumaroyl- and caffeoyl-CoA with K(m)-values of 20 and 33-mu-M, respectively. Only R(+)-stereoisomers of the hydroxyphenyllactic acid are accepted by RAS. For 4-hydroxy- and 3,4-dihydroxyphenyllactic acid the K(m)-values were 0.17 and 0.37 mM, respectively. The RAS reaction was inhibited by 4-hydroxymercuribenzoate, the S(-)-stereoisomer of 3,4-dihydroxyphenyllactic acid, hydroxyphenylpyruvates and rosmarinic acid, whereas cinnamic acids were not inhibitory. Coenzyme A showed a non-competitive inhibition. The reverse reaction of RAS, the splitting of rosmarinic acid with help of coenzyme A into caffeoyl-CoA and 3,4-dihydroxyphenyllactate, was readily detected. The kinetic data for this reaction are K(m)-values of 310-mu-M for coenzyme A and 15-mu-M for rosmarinic acid.
引用
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页码:2877 / 2881
页数:5
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