STIMULATION OF THE PHAGE-LAMBDA PL PROMOTER BY INTEGRATION HOST FACTOR REQUIRES THE CARBOXY TERMINUS OF THE ALPHA-SUBUNIT OF RNA-POLYMERASE

被引：45

作者：

GILADI, H ^{[1
]}

IGARASHI, K ^{[1
]}

ISHIHAMA, A ^{[1
]}

OPPENHEIM, AB ^{[1
]}

机构：

[1] NATL INST GENET,DEPT MOLEC GENET,MISHIMA,SHIZUOKA 411,JAPAN

来源：

JOURNAL OF MOLECULAR BIOLOGY | 1992年 / 227卷 / 04期

关键词：

IHF; TRANSCRIPTION INITIATION; RNA POLYMERASE; PHAGE-LAMBDA; ESCHERICHIA-COLI;

D O I：

10.1016/0022-2836(92)90514-K

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Escherichia coli integration host factor (IHF) binds with high affinity to two tandem IHF consensus sequences located upstream from the pL promoter of bacteriophage lambda. IHF was shown to stimulate transcription initiation from the pL promoter by increasing close complex formation (KB). We show here, by the use of reconstituted mutant RNA polymerases, that the C-terminal portion of the α subunit of RNA polymerase plays an essential role in the stimulation of transcription by IHF. Our results are in agreement with the hypothesis that IHF, like the cAMP-CRP activator, increases the affinity of RNA polymerase to the promoter by protein-protein interaction. © 1992.

引用

页码：985 / 990

页数：6

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