ON-COLUMN SAMPLE GATING FOR HIGH-SPEED CAPILLARY ZONE ELECTROPHORESIS

被引：174

作者：

MONNIG, CA ^{[1
]}

JORGENSON, JW ^{[1
]}

机构：

[1] UNIV N CAROLINA,DEPT CHEM,CHAPEL HILL,NC 27599

来源：

ANALYTICAL CHEMISTRY | 1991年 / 63卷 / 08期

关键词：

D O I：

10.1021/ac00008a013

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

High-speed zone electrophoresis in a fused-silica capillary is described. Elevated electric fields and short capillary lengths allow a mixture of fluorescein isothiocyanate (FITC) labeled amino acids to be separated in times as short as 1.5 s. Formation of the analyte zone at the head of the capillary is controlled by laser-induced photolysis of a tagging reagent. This gating procedure allows rapid and automated introduction of sample into the capillary. Ultimately, Joule heating of the buffer limits the speed and efficiency of the separation.

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页码：802 / 807

页数：6

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