PURIFICATION AND PROPERTIES OF ZINC-METALLOPHOSPHOLIPASE-C FROM PSEUDOMONAS-FLUORESCENS

被引：11

作者：

CREVEL, I ^{[1
]}

SALLY, U ^{[1
]}

CARNE, A ^{[1
]}

KATAN, M ^{[1
]}

机构：

[1] INST CANC RES, CHESTER BEATTY LABS, LONDON SW3 6JB, ENGLAND

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 1994年 / 224卷 / 03期

关键词：

D O I：

10.1111/j.1432-1033.1994.00845.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Phospholipase C produced by Pseudomonas fluorescens, isolated as a laboratory contaminant, has been purified to apparent homogeneity by ammonium sulphate fractionation, anion-exchange and size-exclusion chromatographies. The apparent molecular mass of the purified polypeptide was 39.5 kDa. Purified preparations of phospholipase C were used to characterize its enzymic properties and to obtain amino acid sequence of the N-terminus of the molecule. The P. fluorescens phospholipase C hydrolysed PtdEtn, PtdCho and PtdSer (PtdEtn > PtdCho much greater than PtdSer) and was relatively thermostable. The enzyme was inactivated in the presence of chelating agent o-phenanthroline and the activity restored after addition of zinc. Properties of this enzyme and in particular the requirement for zinc ions for the activity, revealed similarity with the well characterised Bacillus cereus phospholipase C. Similarities with other bacterial and mammalian enzymes reported to be related to the B. cereus type are discussed.

引用

页码：845 / 852

页数：8

共 30 条

[11] A BACILLUS-CEREUS CYTOLYTIC DETERMINANT, CEREOLYSIN-AB, WHICH COMPRISES THE PHOSPHOLIPASE-C AND SPHINGOMYELINASE GENES - NUCLEOTIDE-SEQUENCE AND GENETIC-LINKAGE [J].

GILMORE, MS ;

CRUZRODZ, AL ;

LEIMEISTERWACHTER, M ;

KREFT, J ;

GOEBEL, W .

JOURNAL OF BACTERIOLOGY, 1989, 171 (02) :744-753

[12] BACTERIAL-FLORA IN BOTTLED UNCARBONATED MINERAL DRINKING-WATER [J].

GONZALEZ, C ;

GUTIERREZ, C ;

GRANDE, T .

CANADIAN JOURNAL OF MICROBIOLOGY, 1987, 33 (12) :1120-1125

[13] CRYSTAL-STRUCTURE OF PHOSPHOLIPASE-C FROM BACILLUS-CEREUS COMPLEXED WITH A SUBSTRATE-ANALOG [J].

HANSEN, S ;

HOUGH, E ;

SVENSSON, LA ;

WONG, YL ;

MARTIN, SF .

JOURNAL OF MOLECULAR BIOLOGY, 1993, 234 (01) :179-187

[14] HIGH-RESOLUTION (1.5 A) CRYSTAL-STRUCTURE OF PHOSPHOLIPASE-C FROM BACILLUS-CEREUS [J].

HOUGH, E ;

HANSEN, LK ;

BIRKNES, B ;

JYNGE, K ;

HANSEN, S ;

HORDVIK, A ;

LITTLE, C ;

DODSON, E ;

DEREWENDA, Z .

NATURE, 1989, 338 (6213) :357-360

[15] KINETICS OF THE HYDROLYSIS OF MONODISPERSED AND MICELLAR PHOSPHATIDYLCHOLINES CATALYZED BY A PHOSPHOLIPASE-C FROM BACILLUS-CEREUS [J].

IKEDA, K ;

INOUE, S ;

AMASAKI, C ;

TESHIMA, K ;

IKEZAWA, H .

JOURNAL OF BIOCHEMISTRY, 1991, 110 (01) :88-95

[16] PSEUDOMONAS-FLUORESCENS BACTEREMIA FROM BLOOD-TRANSFUSION [J].

KHABBAZ, RF ;

ARNOW, PM ;

HIGHSMITH, AK ;

HERWALDT, LA ;

CHOU, T ;

JARVIS, WR ;

LERCHE, NW ;

ALLEN, JR .

AMERICAN JOURNAL OF MEDICINE, 1984, 76 (01) :62-68

[17] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].

LAEMMLI, UK .

NATURE, 1970, 227 (5259) :680-+

[18] PHOSPHOLIPASE-C MEDIATED HYDROLYSIS OF PHOSPHATIDYLCHOLINE IS AN IMPORTANT STEP IN PDGF-STIMULATED DNA-SYNTHESIS [J].

LARRODERA, P ;

CORNET, ME ;

DIAZMECO, MT ;

LOPEZBARAHONA, M ;

DIAZLAVIADA, I ;

GUDDAL, PH ;

JOHANSEN, T ;

MOSCAT, J .

CELL, 1990, 61 (06) :1113-1120

[19] PHOSPHOLIPASE-C AND HEMOLYTIC ACTIVITIES OF CLOSTRIDIUM-PERFRINGENS ALPHA-TOXIN CLONED IN ESCHERICHIA-COLI - SEQUENCE AND HOMOLOGY WITH A BACILLUS-CEREUS PHOSPHOLIPASE-C [J].

LESLIE, D ;

FAIRWEATHER, N ;

PICKARD, D ;

DOUGAN, G ;

KEHOE, M .

MOLECULAR MICROBIOLOGY, 1989, 3 (03) :383-392

[20] METAL-ION DEPENDENCE OF PHOSPHOLIPASE-C FROM BACILLUS-CEREUS [J].

LITTLE, C ;

OTNASS, AB .

BIOCHIMICA ET BIOPHYSICA ACTA, 1975, 391 (02) :326-333

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