EFFECT OF ENZYME RATIO AND ENZYME LOADING ON THE PERFORMANCE OF A BIENZYMATIC ELECTROCHEMICAL PHOSPHATE BIOSENSOR

被引:29
作者
DURSO, EM
COULET, PR
机构
[1] Laboratoire de Génie Enzymatique, EP 19 CNRS, Université Claude Bernard Lyon 1, 69622 Villeurbanne Cedex, 43 Boulevard du
关键词
AMPEROMETRY; BIOSENSORS; ENZYMATIC METHODS; ENZYME ELECTRODES; MEMBRANE ELECTRODES; PHOSPHATE;
D O I
10.1016/0003-2670(93)85012-9
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A membrane-bound bienzyme system involving purine nucleoside phosphorylase and xanthine oxidase for the design of an electrochemical biosensor for phosphate detection was studied. The effects on the performance of the biosensor of both the ratio of enzyme activities and the absolute activities in the enzyme loading solution were determined. Optimization of these parameters enabled the linear dynamic range and sensitivity to be improved. The relationship between sensitivity and immobilized activity was also investigated with the sensor equipped with xanthine oxidase alone. The resulting characteristics of the biosensor were compared with those obtained with either glucose oxidase or choline oxidase bound to the membrane.
引用
收藏
页码:535 / 542
页数:8
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