RECOMBINANT 46-KILODALTON SURFACE-ANTIGEN (P46) OF MYCOPLASMA-HYOPNEUMONIAE EXPRESSED IN ESCHERICHIA-COLI CAN BE USED FOR EARLY SPECIFIC DIAGNOSIS OF MYCOPLASMAL PNEUMONIA OF SWINE BY ENZYME-LINKED-IMMUNOSORBENT-ASSAY

被引：27

作者：

FUTO, S

SETO, Y

OKADA, M

SATO, S

SUZUKI, T

KAWAI, K

IMADA, Y

MORI, Y

机构：

[1] GIFU UNIV, FAC AGR, DEPT BIOTECHNOL, GIFU 50111, JAPAN

[2] GIFU UNIV, UNITED GRAD SCH AGR SCI, GIFU 50111, JAPAN

[3] NIPPON FLOUR MILLS CO LTD, CENT LAB, ATSUGI, KANAGAWA 243, JAPAN

[4] ZEN NOH INST FEED & LIVESTOCK RES, SAKURA, CHIBA 285, JAPAN

[5] NATL INST ANIM HLTH, TSUKUBA, IBARAKI 305, JAPAN

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1995年 / 33卷 / 03期

关键词：

D O I：

10.1128/JCM.33.3.680-683.1995

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The 46-kDa surface antigen (P46) is the early and species-specific immunogenic protein of Mycoplasma hyopneumoniae. Three TGA codons encoding tryptophan in the P46 gene were replaced with TGG by an in vitro mutagenesis technique, The mutated P46 gene was expressed in Escherichia coli by using the chelating peptide tag system. The purified recombinant P46 was successfully used in an enzyme-linked immunosorbent assay for detection of antibodies against M. hyopneumoniae in swine serum. It did not cross-react with sera from swine infected with Mycoplasma flocculate, Mycoplasma hyorhinis, or Mycoplasma hyosynoviae. With this method, mycoplasmal pneumonia of swine was detectable within 2 weeks after infection.

引用

页码：680 / 683

页数：4

共 23 条

[11]

MIZUSHIMA S, 1984, MOL CELL BIOCHEM, V60, P5

[12]

MORI Y, 1987, ISRAEL J MED SCI, V23, P657

[13] IMMUNOBLOTTING ANALYSIS OF ANTIBODY-RESPONSE IN SWINE EXPERIMENTALLY INOCULATED WITH MYCOPLASMA-HYOPNEUMONIAE [J].