HUMAN MONOCLONAL FAB FRAGMENTS DERIVED FROM A COMBINATORIAL LIBRARY BIND TO RESPIRATORY SYNCYTIAL VIRUS-F GLYCOPROTEIN AND NEUTRALIZE INFECTIVITY

被引：168

作者：

BARBAS, CF

CROWE, JE

CABABA, D

JONES, TM

ZEBEDEE, SL

MURPHY, BR

CHANOCK, RM

BURTON, DR

机构：

[1] RW JOHNSON PHARMACEUT RES INST, SAN DIEGO, CA 92121 USA

[2] NIAID, INFECT DIS LAB, BETHESDA, MD 20892 USA

[3] SCRIPPS RES INST, DEPT MOLEC BIOL, LA JOLLA, CA 92037 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1992年 / 89卷 / 21期

关键词：

ANTIBODY REPERTOIRES; PASSIVE IMMUNIZATION; FILAMENTOUS PHAGE; PHAGE SURFACE EXPRESSION;

D O I：

10.1073/pnas.89.21.10164

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Respiratory syncytial virus (RSV) is the most important cause, throughout the world, of severe viral lower respiratory tract illness in young children. Antibodies are known to mediate resistance to RSV infection and illness. We have isolated a number of human monoclonal Fab fragments to RSV F glycoprotein from a combinatorial antibody library expressed on the surface of phage. One of these neutralized a wide range of virus isolates, 10 subgroup A and 9 subgroup B isolates, with a titer (60 % neutralization) of almost-equal-to 0.1-1.0 mug/ml. Another Fab neutralized diverse isolates at a concentration somewhat higher. These human Fab fragments show great promise for use in the prophylaxis or therapy of serious RSV lower respiratory tract disease. For intramuscular or intravenous administration, whole antibodies will be required, whereas for aerosol application, F(ab')2 or Fab fragments may suffice.

引用

页码：10164 / 10168

页数：5