EXPRESSION OF GLUTAMATE TRANSPORTERS IN CULTURED GLIAL-CELLS

被引：112

作者：

KONDO, K

HASHIMOTO, H

KITANAKA, J

SAWADA, M

SUZUMURA, A

MARUNOUCHI, T

BABA, A

机构：

[1] OSAKA UNIV,FAC PHARMACEUT SCI,DEPT PHARMACOL,SUITA,OSAKA 565,JAPAN

[2] FUJITA HLTH UNIV,INST COMPREHENS MED SCI,DIV CELL BIOL,TOYOAKE,AICHI 47011,JAPAN

[3] FUJITA HLTH UNIV,SCH MED,DEPT NEUROL,TOYOAKE,AICHI 47011,JAPAN

来源：

NEUROSCIENCE LETTERS | 1995年 / 188卷 / 02期

基金：

日本学术振兴会;

关键词：

GLUTAMATE TRANSPORTER; GLUTAMATE ASPARTATE TRANSPORTER; ASTROCYTES; MICROGLIA; OLIGODENDROCYTES;

D O I：

10.1016/0304-3940(95)11408-O

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Expression of mRNAs for glutamate transporter (GLT-1) and glutamate aspartate transporter (GLAST) was investigated in three different types of purified glial cells by the reverse transcriptase-polymerase chain reaction (RT-PCR). Cultured astrocytes, oligodendrocytes, and microglia expressed mRNAs for GLAST and GLT-1; mRNA for GLAST was expressed more prominently than that for GLT-1 in astrocytes. Oligodendrocytes and microglia expressed mRNAs for both GLT-1 and GLAST equally, but the expression in microglia was not prominent, suggesting glutamate uptake is not essential in microglia. In astrocytes cultured from different brain regions, GLAST mRNA was equally expressed. GLT-1 mRNA was also detected in these astrocytes, but the expression level was lower than that of GLAST.

引用

页码：140 / 142

页数：3

共 9 条

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