MOLECULAR-CLONING AND FUNCTIONAL-CHARACTERIZATION OF A HUMAN VIP RECEPTOR FROM SUP-T1 LYMPHOBLASTS

被引：149

作者：

SVOBODA, M

TASTENOY, M

VANRAMPELBERGH, J

GOOSSENS, JF

DENEEF, P

WAELBROECK, M

ROBBERECHT, P

机构：

[1] Department of Biochemistry and Nutrition, Université Libre de Bruxelles, Brussels B-1070, Building G/E, CP 611

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1994年 / 205卷 / 03期

关键词：

D O I：

10.1006/bbrc.1994.2852

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have cloned and sequenced a cDNA isolated from a human SUP-T1 lymphoblast cell line library. It encoded a 457 amino acids protein having 87% identity with the rat PACAP type II, VIP2 receptor. Chinese hamster ovary (CHO) cells stably transfected with cloned cDNA expressed a specific binding of I-125[Acetyl-His(1)]PACAP-27. This binding was inhibited by GTP, and by the peptides helodermin, VIP, PACAP-27 and PACAP-38 that also stimulated adenylate cyclase activity. The order of potency was PACAP-38 > VIP greater than or equal to helodermin greater than or equal to PACAP-27. Comparison of the results in two cell lines expressing different receptor densities suggested that helodermin and PACAP-38 had a higher intrinsic activity than VIP and PACAP-27. (C) 1994 Academic Press, Inc.

引用

页码：1617 / 1624

页数：8

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