INCREASING GLUCOSE DETERMINATION RANGE BY FLOW-INJECTION ANALYSIS (FIA) USING GLUCOSE-OXIDASE IMMOBILIZED ON POLYANILINE

被引：20

作者：

LEITE, V

DASILVA, VL

AZEVEDO, WM

MELO, EHM

LIMA, JL

机构：

[1] UNIV FED PERNAMBUCO, DEPT BIOQUIM, AV PROF MORAES REGO, BR-50730910 RECIFE, PE, BRAZIL

[2] UNIV FED PERNAMBUCO, LIKA, IMUNOPATOL KEIZO ASAMI LAB, BR-50730910 RECIFE, PE, BRAZIL

[3] UNIV FED PERNAMBUCO, CT, DEPT ENGN QUIM, BR-50730910 RECIFE, PE, BRAZIL

[4] UNIV FED PERNAMBUCO, CCEN, DEPT QUIM FUNDAMENTAL, LQES, BR-50730910 RECIFE, PE, BRAZIL

来源：

BIOTECHNOLOGY TECHNIQUES | 1994年 / 8卷 / 02期

关键词：

D O I：

10.1007/BF00152854

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A Flow Injection Analysis (FIA) for glucose using glucose oxidase (E.C.1.1.3.4) was developed. The enzyme was immobilised on polyaniline chemically synthetized on internal surface of silicon tube (2.0 mm of diameter and length from 1 to 5 meters), using glutaraldehyde as a bifunctional agent. The system was able to measure glucose at levels from 5 to 500mM with a response time of 2min using 250mul of sample. The system has been operated satisfactorily for one month with more than 300 assays with loss of 60% of activity.

引用

页码：133 / 136

页数：4

共 12 条

[1] AMYLASES, ALPHA AND BETA [J].