SPIN-TRAPPED RADICALS - DETERMINATION BY LC-TSP-MS AND LC-ESI-MS

被引：24

作者：

PARKER, CE ^{[1
]}

IWAHASHI, H ^{[1
]}

TOMER, KB ^{[1
]}

机构：

[1] NIEHS,MOLEC BIOPHYS LAB,POB 12233,RES TRIANGLE PK,NC 27709

来源：

JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY | 1991年 / 2卷 / 05期

关键词：

D O I：

10.1016/1044-0305(91)85007-S

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The 4-POBN[alpha-(4-pyridyl-1-oxide)-N-tert-butyl-nitrone] radical adducts of ethyl and pentyl radicals were determined by a combination of high performance liquid chromatography (HPLC) combined with electron paramagnetic resonance (EPR) with HPLC-electrospray (ESI)-mass spectrometry and HPLC-thermospray (TSP)-MS. The identification of the peak corresponding to the spin-trapped radical was done by performing HPLC-EPR under the same chromatographic conditions as the HPLC-MS. The radical adducts could be determined by both techniques, even though for ESI only 12-mu-L/min of the total 1 mL/min HPLC flow rate could be directed into the ion source.

引用

页码：413 / 418

页数：6

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