HIGHLY SENSITIVE DETECTION OF ALLERGEN USING BACTERIAL MAGNETIC PARTICLES

被引：66

作者：

NAKAMURA, N ^{[1
]}

MATSUNAGA, T ^{[1
]}

机构：

[1] TOKYO UNIV AGR & TECHNOL,DEPT BIOTECHNOL,KOGANEI,TOKYO 184,JAPAN

来源：

ANALYTICA CHIMICA ACTA | 1993年 / 281卷 / 03期

关键词：

FLUOROMETRY; IMMUNOASSAY; ALLERGENS; BACTERIAL MAGNETIC PARTICLES; MAGNETIC BACTERIA;

D O I：

10.1016/0003-2670(93)85018-F

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A fluoroimmunoassay method using bacterial magnetic particles for the highly sensitive detection of allergen was developed. Fluorescein isothiocyanate (FITC)-conjugated IgE antibody was immobilized on bacterial magnetic particles using a heterobifunctional reagent, N-succinimidyl 3-(2-pyridyidithio)propionate. The decrease in fluorescence intensity on aggregation of FITC-labelled bacterial magnetic particle conjugates was determined by spectrofluorimetry. The relative fluorescence intensity decreased with increasing allergen concentration. A linear relationship was obtained between the relative fluorescence intensity and allergen concentration in the range of 0.5-100 pg ml-1.

引用

页码：585 / 589

页数：5