A SIMPLE AND RAPID METHOD FOR SEQUENCING THE ENTIRE CODING REGION OF THE HUMAN PRION PROTEIN (PRP) GENE

被引：4

作者：

BOUSSIN, F

JAEGLY, A

DESLYS, JP

DORMONT, D

机构：

[1] Laboratoire de Neuropathologie expérimentale et Neurovirologie DPTE, DSV, CEA, 92 265 Fontenay-aux-Roses cedex

来源：

JOURNAL OF NEUROSCIENCE METHODS | 1994年 / 54卷 / 01期

关键词：

SUBACUTE SPONGIFORM TRANSMISSIBLE ENCEPHALOPATHY; PRION PROTEIN; POLYMORPHISM; NUCLEOTIDE SEQUENCING; POLYMERASE CHAIN REACTION;

D O I：

10.1016/0165-0270(94)90164-3

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The accumulation in brain of the 'prion protein' (PrP), a host-encoded sialoglycoprotein, is the unique specific molecular marker of subacute spongiform transmissible encephalopathies (SSTE). Furthermore, the primary sequence of the PrP gene (PRNP) seems to contain some genetic determinants of great importance in the development of SSTE. Here we present a simple and rapid polymerase chain reaction (PCR)-based method for direct sequencing of the entire coding sequence of the PrP gene, PRNP, in patients. The ability to determine sequences of both alleles of the PRNP gene is demonstrated in the analysis of 3 patients previously established as codon 129 heterozygotes by the use allele-specific oligonucleotide hybridization method.

引用

页码：103 / 107

页数：5

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