ENZYME LEAKAGE IN MUSCLE-TISSUE OF RAINBOW-TROUT (ONCORHYNCHUS-MYKISS) RELATED TO VARIOUS THAWING TREATMENTS

被引:11
作者
NILSSON, K
EKSTRAND, B
机构
[1] SIK - The Swedish Institute for Food Research, Göteborg, S-40229
来源
ZEITSCHRIFT FUR LEBENSMITTEL-UNTERSUCHUNG UND-FORSCHUNG | 1994年 / 198卷 / 03期
关键词
D O I
10.1007/BF01192605
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
Frozen rainbow trout (Oncorhynchus mykiss) was thawed so that different tempering times, followed by different duration times in the temperature intervall called the latent zone (LZ), were obtained. The different thawing treatments resulted in different effects on the muscle membrane system. To estimate the resulting tissue damage, the leakage of the lysosomal enzymes alpha-glucosidase (E.C. 3.2.1.20) and beta-N-glucosaminidase (E.C. 3.2.1.30) was measured as the relationship between the enzyme activity in centrifuged tissue fluid (CTF) and in total homogenate. The volume and protein content of the CTF were also measured. The tempering rate seemed to influence the enzyme leakage more than different duration times in the LZ. Slow tempering increased the enzyme leakage more than fast tempering. Samples taken 1 h in the LZ showed an increased activity compared with samples taken directly after completed tempering. No significant further increase was found in samples taken after 10 or 24 h in the LZ, either for the rapidly or the slowly tempered group.
引用
收藏
页码:253 / 257
页数:5
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